Use of anti-inflammatory medication in healthy athletes - no pain, no gain?
Mackey, A. L., dec. 2007, I: Scandinavian journal of medicine & science in sports. 17, 6, s. 613-4 2 s.
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Mackey, A. L., dec. 2007, I: Scandinavian journal of medicine & science in sports. 17, 6, s. 613-4 2 s.
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Rasmussen, J. N., Gislason, G. H., Rasmussen, S., Abildstrom, S. Z., Schramm, T. K., Køber, L., Diderichsen, F., Osler, M., Torp-Pedersen, C. & Madsen, M., dec. 2007, I: Journal of Epidemiology and Community Health. 61, 12, s. 1091-1097 7 s.
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Romero-Tabarez, M., Jansen, R., Sylla, M., Lünsdorf, H., Häussler, S., Santosa, D. A., Timmis, K. N. & Molinari, G., maj 2006, I: Antimicrobial Agents and Chemotherapy. 50, 5, s. 1701-9 9 s.
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O'Neill, S., Greenberg, R. K., Resch, T., Bathurst, S., Fleming, D., Kashyap, V., Lyden, S. P. & Clair, D., jun. 2006, I: Journal of Vascular Surgery. 43, 6, s. 1103-10 8 s.
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Khoo, S.-K., Zhang, G., Backer, V., Porsbjerg, C., Nepper-Christensen, S., Creegan, R., Baynam, G., de Klerk, N., Rossi, G. A., Hagel, I., Di Prisco, M. C., Lynch, N., Britton, J., Hall, I., Musk, A. W., Goldblatt, J., Le Souëf, P. N. & Greenlandic Population Study Group, sep. 2006, I: The Journal of allergy and clinical immunology. 118, 3, s. 627-34 8 s.
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Maribo, T., Lauritsen, J., Waehrens, E., Poulsen, I. & Hesselbo, B., 2006, I: Ugeskrift for Laeger. 168, 34, s. 2790-2 3 s.
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Bidragets oversatte titel | Barthel Index for evaluation of function: a Danish consensus on its use |
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Originalsprog | Dansk |
Tidsskrift | Ugeskrift for Laeger |
Vol/bind | 168 |
Udgave nummer | 34 |
Sider (fra-til) | 2790-2 |
Antal sider | 3 |
ISSN | 0041-5782 |
Status | Udgivet - 2006 |
Milman, N., Hansen, A., van Overeem Hansen, T., Byg, K-E. & Nielsen, O. H., 16 okt. 2006, I: Ugeskrift for Laeger. 168, 42, s. 3631-3 3 s.
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Olgaard, K. & Lewin, E., maj 2006, I: Clinical journal of the American Society of Nephrology : CJASN. 1, 3, s. 367-73 7 s.
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Björkqvist, M., Petersén, A., Nielsen, J., Ecker, D., Mulder, H., Hayden, M. R., Landwehrmeyer, B., Brundin, P. & Leavitt, B. R., jul. 2006, I: Clinical Genetics. 70, 1, s. 78-9 2 s.
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Resch, T. A., Greenberg, R. K., Lyden, S. P., Clair, D. G., Krajewski, L., Kashyap, V. S., O'Neill, S., Svensson, L. G., Lytle, B. & Ouriel, K., aug. 2006, I: Journal of endovascular therapy : an official journal of the International Society of Endovascular Specialists. 13, 4, s. 481-9 9 s.
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Kruse, M., Hochstrasser, S., Zwisler, A-D. O. & Kjellberg, J., 2006, I: International Journal of Technology Assessment in Health Care. 22, 4, s. 478-83 6 s.
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Eriksen, J., Sjøgren, P., Bruera, E., Ekholm, O. & Rasmussen, N. K., nov. 2006, I: Pain. 125, 1-2, s. 172-9 8 s.
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Ortis, F., Cardozo, A. K., Crispim, D., Störling, J., Mandrup-Poulsen, T. & Eizirik, D. L., 2006, I: Molecular Endocrinology. 20, 8, s. 1867-1879 13 s.
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Henriksen, K., Sørensen, M. G., Nielsen, R. H., Gram, J., Schaller, S., Dziegiel, M. H., Everts, V., Bollerslev, J. & Karsdal, M. A., jan. 2006, I: Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 21, 1, s. 58-66 9 s.
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UNLABELLED: Osteoclasts degrade bone matrix by secretion of hydrochloric acid and proteases. We studied the processes involved in the degradation of the organic matrix of bone in detail and found that lysosomal acidification is involved in this process and that MMPs are capable of degrading the organic matrix in the absence of cathepsin K.
INTRODUCTION: Osteoclasts resorb bone by secretion of acid by the vacuolar H+-adenosine triphosphatase (V-ATPase) and the chloride channel ClC-7, followed by degradation of the matrix, mainly collagen type I, by cathepsin K and possibly by matrix metalloproteinases (MMPs). However, the switch from acidification to proteolysis and the exact roles of both the ion transporters and the proteinases still remain to be studied.
MATERIALS AND METHODS: We isolated CD14+ monocytes from human peripheral blood from either controls or patients with autosomal dominant osteopetrosis type II (ADOII) caused by defective ClC-7 function and cultured them in the presence of RANKL and macrophage-colony stimulating factor (M-CSF) to generate osteoclasts. We decalcified cortical bovine bone slices and studied the osteoclasts with respect to morphology, markers, and degradation of the decalcified matrix in the presence of various inhibitors of osteoclast acidification and proteolysis, using normal calcified bone as a reference.
RESULTS: We found that ADOII osteoclasts not only have reduced resorption of the calcified matrix, but also 40% reduced degradation of the organic phase of bone. We found that both acidification inhibitors and cathepsin K inhibitors reduced degradation of the organic matrix by 40% in normal osteoclasts, but had no effect in the ADOII osteoclasts. Furthermore, we showed that inhibition of MMPs leads to a 70% reduction in the degradation of the organic bone matrix and that MMPs and cathepsin K have additive effects. Finally, we show that osteoclastic MMPs mediate release of the carboxyterminal telopeptide of type I collagen (ICTP) fragment in the absence of cathepsin K activity, and therefore, to some extent, are able to compensate for the loss of cathepsin K activity.
CONCLUSIONS: These data clearly show that osteoclastic acidification of the lysosomes plays a hitherto nonrecognized role in degradation of the organic matrix. Furthermore, these data shed light on the complicated interplay between acidification dependent and independent proteolytic processes, mediated by cathepsin K and the MMPs, respectively.
Originalsprog | Engelsk |
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Tidsskrift | Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research |
Vol/bind | 21 |
Udgave nummer | 1 |
Sider (fra-til) | 58-66 |
Antal sider | 9 |
ISSN | 0884-0431 |
DOI | |
Status | Udgivet - jan. 2006 |
Kjaer, M., Suetta, C. & Tønnesen, H., 4 dec. 2006, I: Ugeskrift for Laeger. 168, 49, s. 4322-4 3 s.
Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › peer review
Bidragets oversatte titel | [The physically-inactive surgical patient] |
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Originalsprog | Dansk |
Tidsskrift | Ugeskrift for Laeger |
Vol/bind | 168 |
Udgave nummer | 49 |
Sider (fra-til) | 4322-4 |
Antal sider | 3 |
Status | Udgivet - 4 dec. 2006 |
Sjøgren, P. & Rindom, H., 4 dec. 2006, I: Ugeskrift for Laeger. 168, 49, s. 4317-9 3 s.
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Tønnesen, H. & Møller, A., 4 dec. 2006, I: Ugeskrift for Laeger. 168, 49, s. 4293-6 4 s.
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Bidragets oversatte titel | [The smoking and drinking patient] |
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Originalsprog | Dansk |
Tidsskrift | Ugeskrift for Laeger |
Vol/bind | 168 |
Udgave nummer | 49 |
Sider (fra-til) | 4293-6 |
Antal sider | 4 |
Status | Udgivet - 4 dec. 2006 |
Eriksen, J. & Sjøgren, P., 15 maj 2006, I: Ugeskrift for Laeger. 168, 20, s. 1947-50 4 s.
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Sørensen, M. G., Henriksen, K., Dziegiel, M. H., Tankó, L. B. & Karsdal, M. A., aug. 2006, I: D N A and Cell Biology. 25, 8, s. 475-83 9 s.
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Estrogen deficiency arising with the menopause promotes marked acceleration of bone resorption, which can be restored by hormone replacement therapy. The inhibitory effects of estrogen seem to involve indirect cytokine- mediated effects via supporting bone marrow cells, but direct estrogen-receptor mediated effects on the bone-resorbing osteoclasts have also been proposed. Little information is available on whether estrogens modulate human osteoclastogenesis or merely inhibit the functional activity of osteoclasts. To clarify whether estrogens directly modulate osteoclastic activities human CD14+ monocytes were cultured in the presence of M-CSF and RANKL to induce osteoclast differentiation. Addition of 0.1-10 nM 17beta-estradiol to differentiating osteoclasts resulted in a dose-dependent reduction in tartrate resistant acid phosphatase (TRACP) activity reaching 60% at 0.1 nM. In addition, 17beta-estradiol inhibited bone resorption, as measured by the release of the C-terminal crosslinked telopeptide (CTX), by 60% at 0.1 nM, but had no effect on the overall cell viability. In contrast to the results obtained with differentiating osteoclasts, addition of 17beta-estradiol (0.001-10 nM) to mature osteoclasts did not affect bone resorption or TRACP activity. We investigated expression of the estrogen receptors, using immunocytochemistry and Western blotting. We found that ER-alpha is expressed in osteoclast precursors, whereas ER- beta is expressed at all stages, indicating that the inhibitory effect of estrogen on osteoclastogenesis is mediated by ER-alpha for the major part. In conclusion, these results suggest that the in vivo effects of estrogen are mediated by reduction of osteoclastogenesis rather than direct inhibition of the resorptive activity of mature osteoclasts.
Originalsprog | Engelsk |
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Tidsskrift | D N A and Cell Biology |
Vol/bind | 25 |
Udgave nummer | 8 |
Sider (fra-til) | 475-83 |
Antal sider | 9 |
ISSN | 1044-5498 |
DOI | |
Status | Udgivet - aug. 2006 |
Balke, B., Hogardt, M., Schmoldt, S., Hoy, L., Weissbrodt, H. & Häussler, S., jan. 2006, I: European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology. 25, 1, s. 25-30 6 s.
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