Published in 2007

An evaluation of a model for the systematic documentation of hospital based health promotion activities: results from a multicentre study

Tønnesen, H., Christensen, M. E., Groene, O., O'Riordan, A., Simonelli, F., Suurorg, L., Morris, D., Vibe, P., Himel, S. & Hansen, P. E., 1 jan. 2007, I: B M C Health Services Research. 7, s. 145

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

The first step of handling health promotion (HP) in Diagnosis Related Groups (DRGs) is a systematic documentation and registration of the activities in the medical records. So far the possibility and tradition for systematic registration of clinical HP activities in the medical records and in patient administrative systems have been sparse. Therefore, the activities are mostly invisible in the registers of hospital services as well as in budgets and balances.A simple model has been described to structure the registration of the HP procedures performed by the clinical staff. The model consists of two parts; first part includes motivational counselling (7 codes) and the second part comprehends intervention, rehabilitation and after treatment (8 codes).The objective was to evaluate in an international study the usefulness, applicability and sufficiency of a simple model for the systematic registration of clinical HP procedures in day life.
Originalsprog Engelsk
Tidsskrift B M C Health Services Research
Vol/bind 7
Sider (fra-til) 145
ISSN 1472-6963
Status Udgivet - 1 jan. 2007

Blau syndrome-associated mutations in exon 4 of the caspase activating recruitment domain 15 (CARD 15) gene are not found in ethnic Danes with sarcoidosis

Milman, N., Nielsen, F. C., Hviid, T. V. F. & Hansen, T. V. O., dec. 2007, I: The clinical respiratory journal. 1, 2, s. 74-9 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Characterization of osteoclasts derived from CD14+ monocytes isolated from peripheral blood

Sørensen, M. G., Henriksen, K., Schaller, S., Henriksen, D. B., Nielsen, F. C., Dziegiel, M. H. & Karsdal, M. A., 2007, I: Journal of Bone and Mineral Metabolism. 25, 1, s. 36-45 10 s.

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Bone resorption is solely mediated by osteoclasts. Therefore, a pure osteoclast population is of high interest for the investigation of biological aspects of the osteoclasts, such as the direct effect of growth factors and hormones, as well as for testing and characterizing inhibitors of bone resorption. We have established a pure, stable, and reproducible system for purification of human osteoclasts from peripheral blood. We isolated CD14-positive (CD14+) monocytes using anti-CD14-coated beads. After isolation, the monocytes are differentiated into mature osteoclasts by stimulation with macrophage colony-stimulating factor (M-CSF) and receptor activator of nuclear factor kappaB ligand (RANKL). Osteoclast formation was only observed in the CD14+ population, not in the CD14- population, and only in the presence of both M-CSF and RANKL, confirming that the CD14+ system is a pure population of osteoclast precursors. No expression of osteoclast markers was observed in the absence of RANKL, whereas RANKL dose-dependently induced the expression of cathepsin K, tartrate-resistant acid phosphatase (TRACP), and matrix metallo proteinase (MMP)-9. Furthermore, morphological characterization of the cells demonstrated that actin rings were only formed in the presence of RANKL. Moreover, the osteoclasts were capable of forming acidic resorption lacunae, and inhibitors of lysosomal acidification attenuated this process. Finally, we measured the response to known bone resorption inhibitors, and found that the osteoclasts were sensitive to these and thereby provided a robust and valid method for interpretation of the effect of antiresorptive compounds. In conclusion, we have established a robust assay for developing osteoclasts that can be used to study several biological aspects of the osteoclasts and which in combination with the resorption marker CTX-I provides a useful tool for evaluating osteoclast function in vitro.

Originalsprog Engelsk
Tidsskrift Journal of Bone and Mineral Metabolism
Vol/bind 25
Udgave nummer 1
Sider (fra-til) 36-45
Antal sider 10
ISSN 0914-8779
Status Udgivet - 2007

Computerized cuff pressure algometry: A new method to assess deep-tissue hypersensitivity in fibromyalgia

Jespersen, A., Dreyer, L., Kendall, S., Graven-Nielsen, T., Arendt-Nielsen, L., Bliddal, H. & Danneskiold-Samsoe, B., sep. 2007, I: Pain. 131, 1-2, s. 57-62 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The aim of this study was to evaluate the use of computerized cuff pressure algometry (CPA) in fibromyalgia (FM) and to correlate deep-tissue sensitivity assessed by CPA with other disease markers of FM. Forty-eight women with FM and 16 healthy age-matched women were included. A computer-controlled, pneumatic tourniquet cuff was placed over the gastrocnemius muscle. The cuff was inflated, and the subject rated the pain intensity continuously on an electronic Visual Analogue Scale (VAS). The subject stopped the inflation at the pressure-pain tolerance and the corresponding VAS-score was determined (pressure-pain limit). The pressure at which VAS firstly exceeded 0 was defined as the pressure-pain threshold. Other disease markers (FM only): Isokinetic knee muscle strength, tenderpoint-count, myalgic score, Beck Depression Inventory, and Fibromyalgia Impact Questionnaire. Student's T-test was used to compare pressure-pain threshold and pressure-pain tolerance and the Mann-Whitney test to compare pressure-pain limit. Pearson's correlation was used to detect linear relationships. Pressure-pain threshold and pressure-pain tolerance assessed by CPA were significantly lower in FM compared to healthy controls. There was no difference in pressure-pain limit. CPA-parameters were significantly correlated to isokinetic muscle strength where more hypersensitivity resulted in lower strength. Pressure-pain threshold and pressure-pain tolerance assessed by CPA were significantly lower in patients with FM indicating muscle hyperalgesia. CPA was associated with knee muscle strength but not with measures thought to be influenced by psychological distress and mood.

Originalsprog Engelsk
Tidsskrift Pain
Vol/bind 131
Udgave nummer 1-2
Sider (fra-til) 57-62
Antal sider 6
ISSN 0304-3959
Status Udgivet - sep. 2007

Differences between allergic and nonallergic rhinitis in a large sample of adolescents and adults

Mølgaard, E., Thomsen, S. F., Lund, T., Pedersen, L., Nolte, H. & Backer, V., sep. 2007, I: Allergy. 62, 9, s. 1033-7 5 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Diminished NO release in chronic hypoxic human endothelial cells

Ostergaard, L., Stankevicius, E., Andersen, M. R., Eskildsen-Helmond, Y., Ledet, T., Mulvany, M. J. & Simonsen, U., nov. 2007, I: American Journal of Physiology: Heart and Circulatory Physiology. 293, 5, s. H2894-903

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

UNLABELLED: Dissolution of the inorganic phase of bone by the osteoclasts mediated by V-ATPase and ClC-7 is a prerequisite for bone resorption. Inhibitors of osteoclastic V-ATPase or ClC-7 are novel approaches for inhibition of osteoclastic bone resorption. By testing natural compounds in acidification assays, diphyllin was identified. We characterized diphyllin with respect to the pharmacological effects on osteoclasts.

INTRODUCTION: Osteoclastic acidification of the resorption lacuna and bone resorption requires activity of both V-ATPase and the chloride channel ClC-7. Inhibition of these processes represents a novel approach for treatment of bone metabolic disorders. We identified diphyllin, a novel inhibitor of V-ATPase, and characterized this natural compound with respect to activity in human osteoclasts.

MATERIALS AND METHODS: Diphyllin was tested in the acid influx assay and V-ATPase assay using bovine chromaffin granules. Human osteoclasts were generated from CD14+ monocytes cultured with macrophage-colony stimulating factor (M-CSF) and RANKL. The effect of diphyllin on lysosomal acidification in human osteoclasts was studied using acridine orange. The effect of diphyllin on bone resorption by osteoclasts was measured as release of C-terminal cross-linked telopeptide of type I collagen (CTX-I) and calcium into the supernatants and by scoring pit area. Osteoclast number, TRACP activity, and cell viability were measured. Furthermore, the effect of diphyllin on bone nodule formation was tested using the mouse osteoblast cell line MC3T3-E1.

RESULTS: In the acid influx assay, diphyllin potently inhibited the acid influx (IC50 = 0.6 nM). We found that diphyllin inhibited V-ATPase with an IC50 value of 17 nM, compared with 4 nM for bafilomycin A1. Moreover, diphyllin dose-dependently inhibited lysosomal acidification in human osteoclasts. Furthermore, we found that diphyllin inhibited human osteoclastic bone resorption measured by CTX-I (IC50 = 14 nM), calcium release, and pit area, despite increasing TRACP activity, numbers of osteoclasts, and cell viability. Finally, diphyllin showed no effect on bone formation in vitro, whereas bafilomycin A1 was toxic.

CONCLUSIONS: We identified a natural compound that potently inhibits V-ATPase and thereby lysosomal acidification in osteoclasts, which leads to abrogation of bone resorption. Because recent studies indicate that inhibition of the osteoclastic acidification leads to inhibition of resorption without inhibiting formation, we speculate that diphyllin is a potential novel treatment for bone disorders involving excessive resorption.

Originalsprog Engelsk
Tidsskrift Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Vol/bind 22
Udgave nummer 10
Sider (fra-til) 1640-8
Antal sider 9
ISSN 0884-0431
Status Udgivet - okt. 2007

Dissolution of the inorganic phase of bone leading to release of calcium regulates osteoclast survival

Nielsen, R. H., Karsdal, M. A., Sørensen, M. G., Dziegiel, M. H. & Henriksen, K., 7 sep. 2007, I: Biochemical and Biophysical Research Communications. 360, 4, s. 834-9 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Osteoclasts are the sole cells possessing the ability to resorb calcified bone matrix. This occurs via secretion of hydrochloric acid mediated by the V-ATPase and the chloride channel ClC-7. Loss of acidification leads to osteopetrosis characterized by ablation of bone resorption and increased osteoclast numbers, indicating increased life span of the osteoclasts. To investigate the role of the inorganic phase of bone with respect to osteoclast life span, we used the V-ATPase inhibitor bafilomycin and the calcium uptake antagonist ryanodine on human osteoclasts cultured on calcified and decalcified bone slices. Bafilomycin inhibited bone resorption and increased osteoclast survival on calcified but not decalcified bones. Ryanodine attenuated calcium uptake and thereby augmented osteoclast survival on calcified bones. In summary, we found that acidification leading to calcium release from bone during resorption controls osteoclast survival, potentially explaining the increased numbers of osteoclasts in patients with osteopetrosis.

Originalsprog Engelsk
Tidsskrift Biochemical and Biophysical Research Communications
Vol/bind 360
Udgave nummer 4
Sider (fra-til) 834-9
Antal sider 6
ISSN 0006-291X
Status Udgivet - 7 sep. 2007

Impairment of the dynamic control of the lumbopelvic complex in LBP has gained increased focus both clinically and experimentally. The objectives of this study were to determine the reliability of inclinometry as a measure of dynamic lumbopelvic control. Lumbopelvic reposition accuracy during pelvic tilts was measured in 39 healthy subjects using an inclinometer attached to the skin at S2 level. The reposition accuracy was measured in sitting, standing and supported standing. Tests were performed three times with a 20 min recess between tests. Only data from the last two test sequences were used in order to account for learning effects. Intraclass correlation coefficients were low for the sitting (0.54) and supported standing positions (0.36). In the standing position, a significant difference between test and retest was observed (P = 0.003) and further reliability analysis was therefore abandoned. It is concluded that inclinometry is not reliable for measuring the dynamic lumbopelvic control in any of the test positions and prior work utilising inclinometry to evaluate dynamic lumbopelvic control should be interpreted with caution.

Originalsprog Engelsk
Tidsskrift European spine journal : official publication of the European Spine Society, the European Spinal Deformity Society, and the European Section of the Cervical Spine Research Society
Vol/bind 16
Udgave nummer 6
Sider (fra-til) 733-40
Antal sider 8
ISSN 0940-6719
Status Udgivet - jun. 2007

Epidemiological and clinical studies on hand eczema in a population-based twin sample

Jørkov, A. L., 2007, Københavns Universitet.

Publikation: Bog/antologi/afhandling/rapportPh.d.-afhandling

Experimental quadriceps muscle pain impairs knee joint control during walking

Henriksen, M., Alkjaer, T., Lund, H., Simonsen, E. B., Graven-Nielsen, T., Danneskiold-Samsøe, B. & Bliddal, H., jul. 2007, I: Journal of applied physiology (Bethesda, Md. : 1985). 103, 1, s. 132-9 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Pain is a cardinal symptom in musculoskeletal diseases involving the knee joint, and aberrant movement patterns and motor control strategies are often present in these patients. However, the underlying neuromuscular mechanisms linking pain to movement and motor control are unclear. To investigate the functional significance of muscle pain on knee joint control during walking, three-dimensional gait analyses were performed before, during, and after experimentally induced muscle pain by means of intramuscular injections of hypertonic saline (5.8%) into vastus medialis (VM) muscle of 20 healthy subjects. Isotonic saline (0.9%) was used as control. Surface electromyography (EMG) recordings of VM, vastus lateralis (VL), biceps femoris, and semitendinosus muscles were synchronized with the gait analyses. During experimental muscle pain, the loading response phase peak knee extensor moments were attenuated, and EMG activity in the VM and VL muscles was reduced. Compressive forces, adduction moments, knee joint kinematics, and hamstring EMG activity were unaffected by pain. Interestingly, the observed changes persisted when the pain had vanished. The results demonstrate that muscle pain modulated the function of the quadriceps muscle, resulting in impaired knee joint control and joint instability during walking. The changes are similar to those observed in patients with knee pain. The loss of joint control during and after pain may leave the knee joint prone to injury and potentially participate in the chronicity of musculoskeletal problems, and it may have clinically important implications for rehabilitation and training of patients with knee pain of musculoskeletal origin.

Originalsprog Engelsk
Tidsskrift Journal of applied physiology (Bethesda, Md. : 1985)
Vol/bind 103
Udgave nummer 1
Sider (fra-til) 132-9
Antal sider 8
ISSN 8750-7587
Status Udgivet - jul. 2007

Guillain-Barré syndrome associated to infection with Borrelia burgdorferi: A case report

von Linstow, M. L. & Andersen, J., 2007, I: Journal of Pediatric Infectious Diseases. 2, 4, s. 247-250 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Increased cancer risk in patients referred to hospital with suspected fibromyalgia

Dreyer, L., Mellemkjaer, L., Kendall, S., Jensen, B., Danneskiold-Samsøe, B. & Bliddal, H., jan. 2007, I: Journal of Rheumatology. 34, 1, s. 201-6 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVE: To analyze whether fibromyalgia (FM) and FM-like symptoms are related to an increased incidence of cancer.

METHODS: We identified 1361 patients referred on suspicion of FM in the period 1984-99 from hospital records. Following the American College of Rheumatology (ACR) criteria, patients were divided into subgroups with and without confirmed FM. The cohort was followed to the end of 1999 and linked to the files of the Danish Cancer Register. Site-specific standardized incidence ratios (SIR) were calculated.

RESULTS: We found no association between FM and cancer in 1132 female patients with confirmed FM at our institution (SIR 1.2, 95% CI 0.8-1.8). In 106 women referred for muscle pain and/or tenderness who did not meet the criteria for FM, an increased overall SIR was observed (SIR 2.5, 95% CI 1.2-4.6), with increased risk for breast cancer (SIR 4.8, 95% CI 1.6-11.3) and lymphatic and hematological cancers (SIR 10.6, 95% CI 1.2-38.2). There were 4 lung cancers in 84 men with confirmed FM (SIR 12.6, 95% CI 3.4-32.4).

CONCLUSION: Neither confirmed FM nor those without confirmed FM predicted cancer. An increased risk of breast cancer was found among those who did not meet the ACR criteria for FM. These patients should be investigated if they develop any new or warning symptoms of malignancy, and treating physicians should be vigilant with screening procedures such as mammography.

Originalsprog Engelsk
Tidsskrift Journal of Rheumatology
Vol/bind 34
Udgave nummer 1
Sider (fra-til) 201-6
Antal sider 6
ISSN 0315-162X
Status Udgivet - jan. 2007

Intra-observer and inter-observer agreement of the manual examination of the lumbar spine in chronic lowback pain

Qvistgaard, E., Rasmussen, J. H., Laetgaard, J., Hecksher-Sørensen, S. & Bliddal, H., feb. 2007, I: European Spine Journal. s. 277-82

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Intratumor genetic heterogeneity of breast carcinomas as determined by fine needle aspiration and TaqMan low density array

Lyng, M. B., Laenkholm, A-V., Pallisgaard, N., Vach, W., Knoop, A., Bak, M. & Ditzel, H. J., 2007, I: Cellular oncology : the official journal of the International Society for Cellular Oncology. 29, 5, s. 361-72 12 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Introduction of new guidelines for emergency patients: motivational counselling among smokers

Backer, V., Nelbom, B. M., Duus, B. R. & Tønnesen, H., 1 jul. 2007, I: Clinical Respiratory Journal. 1, 1, s. 37-41 5 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskning

Smoking is the most important risk factor for morbidity and mortality in hospital patients. Patient contact with the healthcare system should include smoking cessation counselling. Emergency admissions are seldom given this opportunity. Objective: The aim of the study was to illustrate the implementation of motivational counselling in a department of acute surgery.
Originalsprog Engelsk
Tidsskrift Clinical Respiratory Journal
Vol/bind 1
Udgave nummer 1
Sider (fra-til) 37-41
Antal sider 5
ISSN 1752-6981
Status Udgivet - 1 jul. 2007