Published in 2006

Estrogen directly attenuates human osteoclastogenesis, but has no effect on resorption by mature osteoclasts

Sørensen, M. G., Henriksen, K., Dziegiel, M. H., Tankó, L. B. & Karsdal, M. A., aug. 2006, I : D N A and Cell Biology. 25, 8, s. 475-83 9 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Estrogen deficiency arising with the menopause promotes marked acceleration of bone resorption, which can be restored by hormone replacement therapy. The inhibitory effects of estrogen seem to involve indirect cytokine- mediated effects via supporting bone marrow cells, but direct estrogen-receptor mediated effects on the bone-resorbing osteoclasts have also been proposed. Little information is available on whether estrogens modulate human osteoclastogenesis or merely inhibit the functional activity of osteoclasts. To clarify whether estrogens directly modulate osteoclastic activities human CD14+ monocytes were cultured in the presence of M-CSF and RANKL to induce osteoclast differentiation. Addition of 0.1-10 nM 17beta-estradiol to differentiating osteoclasts resulted in a dose-dependent reduction in tartrate resistant acid phosphatase (TRACP) activity reaching 60% at 0.1 nM. In addition, 17beta-estradiol inhibited bone resorption, as measured by the release of the C-terminal crosslinked telopeptide (CTX), by 60% at 0.1 nM, but had no effect on the overall cell viability. In contrast to the results obtained with differentiating osteoclasts, addition of 17beta-estradiol (0.001-10 nM) to mature osteoclasts did not affect bone resorption or TRACP activity. We investigated expression of the estrogen receptors, using immunocytochemistry and Western blotting. We found that ER-alpha is expressed in osteoclast precursors, whereas ER- beta is expressed at all stages, indicating that the inhibitory effect of estrogen on osteoclastogenesis is mediated by ER-alpha for the major part. In conclusion, these results suggest that the in vivo effects of estrogen are mediated by reduction of osteoclastogenesis rather than direct inhibition of the resorptive activity of mature osteoclasts.

Originalsprog Engelsk
Tidsskrift D N A and Cell Biology
Vol/bind 25
Udgave nummer 8
Sider (fra-til) 475-83
Antal sider 9
ISSN 1044-5498
DOI
Status Udgivet - aug. 2006

Impulse-forces during walking are not increased in patients with knee osteoarthritis

Henriksen, M., Simonsen, E. B., Graven-Nielsen, T., Lund, H., Danneskiold-Samsøe, B. & Bliddal, H., aug. 2006, I : Acta Orthopaedica (Print Edition). 77, 4, s. 650-6 7 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

BACKGROUND: Impulsive forces in the knee joint have been suspected to be a co-factor in the development and progression of knee osteoarthritis. We thus evaluated the impulsive sagittal ground reaction forces (iGRF), shock waves and lower extremity joint kinematics at heel strike during walking in knee osteoarthritis (OA) patients and compared them to those in healthy subjects.

SUBJECTS AND METHODS: We studied 9 OA patients and 10 healthy subjects using three-dimensional gait analyses concentrated on the heel strike. Impulse GRF (iGRF) was measured together with peak accelerations (PA) at the tibial tuberosity and sacrum. Sagittal lower extremity joint angles at heel strike were extracted from the gait analyses. As OA is painful and pain might alter movement strategies, the patient group was also evaluated following pain relief by intraarticular lidocaine injections.

RESULTS: The two groups showed similar iGRF, similar tibial and sacral PA, and similar joint angles at heel strike. Following pain relief, the OA patients struck the ground with more extended hip and knee joints and lower tibial PA compared to the painful condition. Although such changes occurred after pain relief, all parameters were within their normal ranges.

INTERPRETATION: OA patients and healthy subjects show similar impulse-forces and joint kinematics at heel strike. Following pain relief in the patient group, changes in tibial PA and in hip and knee joint angles were observed but these were still within the normal range. Our findings make us question the hypothesis that impulse-forces generated at heel strike during walking contribute to progression of OA.

Originalsprog Engelsk
Tidsskrift Acta Orthopaedica (Print Edition)
Vol/bind 77
Udgave nummer 4
Sider (fra-til) 650-6
Antal sider 7
ISSN 1745-3674
DOI
Status Udgivet - aug. 2006

Increased joint loads during walking--a consequence of pain relief in knee osteoarthritis

Henriksen, M., Simonsen, E. B., Alkjaer, T., Lund, H., Graven-Nielsen, T., Danneskiold-Samsøe, B. & Bliddal, H., dec. 2006, I : The Knee. 13, 6, s. 445-50 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Joint pain is a primary symptom in knee osteoarthritis (OA), but the effect of pain and pain relief on the knee joint mechanics of walking is not clear. In this study, the effects of local knee joint analgesia on knee joint loads during walking were studied in a group of knee osteoarthritis patients. A group of healthy subjects was included as a reference group. The joint loads were calculated from standard gait analysis data obtained with standardised walking speed (4 km/h). The gait analyses were performed before and after pain relief by intra-articular injections of 10 mL lidocaine (1%). Pre-injection measurements revealed lower joint loads in the OA group compared to the reference group. Following injections pain during walking decreased significantly and the joint loads increased in the OA group during the late single support phase to a level comparable to the reference group. Although the patients walked with less compressive knee joint forces compared to the reference group, the effects of pain relief may accelerate the degenerative changes.

Originalsprog Engelsk
Tidsskrift The Knee
Vol/bind 13
Udgave nummer 6
Sider (fra-til) 445-50
Antal sider 6
ISSN 0968-0160
DOI
Status Udgivet - dec. 2006

Reproducibility of ultrasound and magnetic resonance imaging measurements of tendon size

Brushøj, C., Henriksen, B. M., Albrecht-Beste, E., Hölmich, P., Larsen, K. & Bachmann Nielsen, M. nov. 2006 I : Acta Radiologica. 47, 9, s. 954-9 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

PURPOSE: To investigate the intra- and inter-tester reproducibility of measurements of the Achilles tendon, tibialis anterior tendon, and the tibialis posterior tendon in football players using ultrasound (US) and magnetic resonance imaging (MRI).

MATERIAL AND METHODS: Eleven asymptomatic football players were examined. Using a standardized US scanning protocol, the tendons were examined by two observers with US for thickness, width, and cross-sectional area. One observer conducted the procedure twice. The subjects also underwent an MRI examination, and the assessment of tendon size was conducted twice by two observers.

RESULTS: The best reproducibility judged by coefficient of variation (CV) and 95% confidence interval was determined for the Achilles tendon on both US and MRI. The variability of US on measurements on the tibialis anterior and tibialis posterior tendons was less than that when using MRI. In 12 out of 18 measurements, there were systematic differences between observers as judged by one-sided F-test.

CONCLUSION: The reproducibility of the three tendons was limited. Precaution should be taken when looking for minor quantitative changes, i.e., training-induced hypertrophy, and when doing so, the Achilles tendon should be used.

Originalsprog Engelsk
Tidsskrift Acta Radiologica
Vol/bind 47
Tidsskriftsnummer 9
Sider (fra-til) 954-9
Antal sider 6
ISSN 0284-1851
DOI
Status Udgivet - nov. 2006

Ultrasound findings in adductor related groin pain

Hölmich, P. & Bachmann Nielsen, M. dec. 2006 I : Ultraschall in der Medizin (Stuttgart, Germany : 1980). 27, 6, s. 509-11 3 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Originalsprog Engelsk
Tidsskrift Ultraschall in der Medizin (Stuttgart, Germany : 1980)
Vol/bind 27
Tidsskriftsnummer 6
Sider (fra-til) 509-11
Antal sider 3
ISSN 0172-4614
DOI
Status Udgivet - dec. 2006

BACKGROUND: High resolution colour Doppler ultrasound shows intratendinous Doppler activity in patients with chronic Achilles tendinopathy. Treatment of this neovascularisation with sclerosing therapy seems to relieve the pain. However, the procedure often has to be repeated.

OBJECTIVE: To investigate the effect of electrocoagulation of the neovessels on tendon pain and tendon vascularity in patients with chronic Achilles tendinopathy.

METHODS: Colour Doppler ultrasound guided electrocoagulation was used on vessels in the ventral portion of the Achilles tendon in 11 patients (seven men, four women, mean age 41 years) with painful chronic mid-portion Achilles tendinosis. A unipolar coagulation device was used.

RESULTS: One patient dropped out after two months (dissatisfied with the results). The remaining 10 patients (91%) were satisfied. These 10 patients were still satisfied at six months of follow up and had returned to their previous level of activity. All 10 patients were "cured" after one treatment. The patient who dropped out received two treatments because of lack of progress. There was significantly reduced pain (Likert pain scale, 0-10) during activity, from a median of 7 (range 4 to 10) at baseline to 0 (0 to 8) at six months' follow up (p<0.005); and at rest, from 1.5 (1 to 5) to 0 (0 to 8) (p = 0.005). In all patients, vascularisation was unchanged at the six months follow up, with no significant change in semiquantitative or quantitative colour scoring.

CONCLUSIONS: Coagulation in the area with vessels entering the tendon appears to be effective treatment for painful chronic mid-tendinous Achilles tendinopathy. No effect on the intratendinous Doppler activity could be detected, suggesting that the effect is independent of changes in blood flow. Localisation of hyperaemia appears to be the key to the pathology and for targeting the treatment. One explanation could be that the effect is obtained by destruction of nerves accompanying the vessels.

Originalsprog Engelsk
Tidsskrift British Journal of Sports Medicine
Vol/bind 40
Udgave nummer 9
Sider (fra-til) 761-6
Antal sider 6
ISSN 0306-3674
DOI
Status Udgivet - sep. 2006

Published in 2005

Acidification of the osteoclastic resorption compartment provides insight into the coupling of bone formation to bone resorption

Karsdal, M. A., Henriksen, K., Sørensen, M. G., Gram, J., Schaller, S., Dziegiel, M. H., Heegaard, A-M., Christophersen, P., Martin, T., Christiansen, C. & Bollerslev, J., feb. 2005, I : American Journal of Pathology. 166, 2, s. 467-76 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Patients with defective osteoclastic acidification have increased numbers of osteoclasts, with decreased resorption, but bone formation that remains unchanged. We demonstrate that osteoclast survival is increased when acidification is impaired, and that impairment of acidification results in inhibition of bone resorption without inhibition of bone formation. We investigated the role of acidification in human osteoclastic resorption and life span in vitro using inhibitors of chloride channels (NS5818/NS3696), the proton pump (bafilomycin) and cathepsin K. We found that bafilomycin and NS5818 dose dependently inhibited acidification of the osteoclastic resorption compartment and bone resorption. Inhibition of bone resorption by inhibition of acidification, but not cathepsin K inhibition, augmented osteoclast survival, which resulted in a 150 to 300% increase in osteoclasts compared to controls. We investigated the effect of inhibition of osteoclastic acidification in vivo by using the rat ovariectomy model with twice daily oral dosing of NS3696 at 50 mg/kg for 6 weeks. We observed a 60% decrease in resorption (DPYR), increased tartrate-resistant acid phosphatase levels, and no effect on bone formation evaluated by osteocalcin. We speculate that attenuated acidification inhibits dissolution of the inorganic phase of bone and results in an increased number of nonresorbing osteoclasts that are responsible for the coupling to normal bone formation. Thus, we suggest that acidification is essential for normal bone remodeling and that attenuated acidification leads to uncoupling with decreased bone resorption and unaffected bone formation.

Originalsprog Engelsk
Tidsskrift American Journal of Pathology
Vol/bind 166
Udgave nummer 2
Sider (fra-til) 467-76
Antal sider 10
ISSN 0002-9440
DOI
Status Udgivet - feb. 2005

Evidence based prevention of hamstring injuries in sport

Petersen, J. & Hölmich, P. jun. 2005 I : British Journal of Sports Medicine. 39, 6, s. 319-23 5 s.

Publikation: Bidrag til tidsskriftReviewForskningpeer review

A common soft tissue injury in sports involving sprinting and jumping is the hamstring strain. A major problem with hamstring strains is the high incidence of reinjury. Muscle injuries can be classified as direct or indirect and are typically grouped into three categories according to severity. A number of potential risk factors have been proposed for hamstring strains. Only a few are evidence based and some are mainly based on theoretical assumptions. There is a lack of clinical research on the effectiveness of rehabilitation programmes for hamstring strains. Although the initial treatment of rest, ice, compression, and elevation is accepted for muscle strains, no consensus exists for their rehabilitation. Not much evidence based research has been carried out on prevention of hamstring strain. To our knowledge only two prospective studies have so far been published. As the injuries are common in football and other sports involving sprinting and jumping, there is a need for further research preferably in the form of randomised controlled trials.

Originalsprog Engelsk
Tidsskrift British Journal of Sports Medicine
Vol/bind 39
Tidsskriftsnummer 6
Sider (fra-til) 319-23
Antal sider 5
ISSN 0306-3674
DOI
Status Udgivet - jun. 2005

Learning effect of isokinetic measurements in healthy subjects, and reliability and comparability of Biodex and Lido dynamometers

Lund, H., Søndergaard, K., Zachariassen, T., Christensen, R., Bülow, P., Henriksen, M., Bartels, E. M., Danneskiold-Samsøe, B. & Bliddal, H., mar. 2005, I : Clinical Physiology and Functional Imaging. 25, 2, s. 75-82 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The aim of this study was to examine the learning effect during a set of isokinetic measurements, to evaluate the reliability of the Biodex System 3 PRO dynamometer, and to compare the Biodex System 3 PRO and the Lido Active dynamometers on both extension and flexion over the elbow and the knee at 60 degrees s(-1). Thirteen (nine women, four men) healthy participants were measured five times using the Biodex and once using the Lido dynamometer. The intervals between the first four tests were 20 min, and 1 week between tests 4 and 5. Between Biodex and Lido measurements there was a 20 min time interval. When comparing the first five measurements (Biodex), no systematic effect over time and an excellent reliability were found with respect to elbow and knee flexion and extension. No difference in muscle strength (Nm) between the Biodex and Lido was observed for knee flexion (P = 0.59), knee extension (P = 0.18) and elbow extension (P = 0.63). However, elbow flexion showed a 14.8% (95% CI: 11.2-18.4%; P = 0.0001) higher peak torque on Biodex. In conclusion, no learning effect was observed and the Biodex proved to be a highly reliable isokinetic dynamometer. A difference was observed when comparing Biodex and Lido on elbow flexion, but the difference did not outrange the expected variation found with a typical isokinetic measurement, which is why both sets of equipment seem applicable in clinical practice.

Originalsprog Engelsk
Tidsskrift Clinical Physiology and Functional Imaging
Vol/bind 25
Udgave nummer 2
Sider (fra-til) 75-82
Antal sider 8
ISSN 1475-0961
DOI
Status Udgivet - mar. 2005

Reduction of the disintegrin and metalloprotease ADAM12 in preeclampsia

Laigaard, J., Sørensen, T., Placing, S., Holck, P., Fröhlich, C., Wøjdemann, K. R., Sundberg, K., Shalmi, A-C., Tabor, A., Nørgaard-Pedersen, B., Ottesen, B., Christiansen, M. & Wewer, U. M., jul. 2005, I : Obstetrics and Gynecology. 106, 1, s. 144-9 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVES: The secreted form of ADAM12 is a metalloprotease that may be involved in placental and fetal growth. We examined whether the concentration of ADAM12 in first-trimester maternal serum could be used as a marker for preeclampsia.

METHODS: We developed a semiautomated, time-resolved, immunofluorometric assay for the quantification of ADAM12 in serum. The assay detected ADAM12 in a range of 78-1248 microg/L. Serum samples derived from women in the first trimester of a normal pregnancy (n = 324) and from women who later developed preeclampsia during pregnancy (n = 160) were obtained from the First Trimester Copenhagen Study. ADAM12 levels were assayed in these serum samples. Serum levels of ADAM12 were converted to multiples of the median (MoM) after log-linear regression of concentration versus gestational age.

RESULTS: Serum ADAM12 levels in women who developed preeclampsia during pregnancy had a mean log MoM of -0.066, which was significantly lower than the mean log MoM of -0.001 for ADAM12 levels observed in serum samples from women with normal pregnancy (P = .008). The mean log MoM was even lower in serum derived from preeclamptic women whose infant's weight at birth was less than 2,500 g (n = 27, mean log MoM of -0.120, P = .053).

CONCLUSION: The maternal serum levels of ADAM12 are significantly lower during the first trimester in women who later develop preeclampsia during pregnancy when compared with levels in women with normal pregnancies. Because the secreted form of ADAM12 cleaves insulin-like growth factor binding protein (IGFBP)-3 and IGFBP-5, the IGF axis may play a role in preeclampsia. ADAM12 may be a useful early marker for preeclampsia.

LEVEL OF EVIDENCE: II-2.

Originalsprog Engelsk
Tidsskrift Obstetrics and Gynecology
Vol/bind 106
Udgave nummer 1
Sider (fra-til) 144-9
Antal sider 6
ISSN 0029-7844
DOI
Status Udgivet - jul. 2005

Published in 2004

Plasma TNF binding capacity profiles during treatment with etanercept in rheumatoid arthritis

Gudbrandsdottir, S., Bliddal, H., Petri, A., Terslev, L., Danneskiold-Samsøe, B., Bjørnhart, B., Bendtzen, K. & Müller, K., 1 jan. 2004, I : Scandinavian Journal of Rheumatology. 33, 6, s. 385-8 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Etanercept (Enbrel) induces a rapid and sustained decline in disease activity in the majority of patients with refractory rheumatoid arthritis (RA). In these patients neutralization of TNFalpha and lymphotoxin (LT), previously termed TNFbeta is mediated by etanercept itself, as well as by naturally occurring soluble TNF receptors. However, the clinical response to treatment with etanercept may vary. Previously, pharmacokinetic studies have focused on the molar concentrations of etanercept, but very little is known about the kinetics of bioactive etanercept in patients treated with etanercept. The purpose of this study was to evaluate kinetics, including inter- and intraindividual variations of the total TNF binding capacity, in RA patients who were on a standard treatment schedule with etanercept.
Originalsprog Engelsk
Tidsskrift Scandinavian Journal of Rheumatology
Vol/bind 33
Udgave nummer 6
Sider (fra-til) 385-8
Antal sider 4
ISSN 0300-9742
Status Udgivet - 1 jan. 2004

Test-retest reliability of trunk accelerometric gait analysis

Henriksen, M., Lund, H., Moe-Nilssen, R., Bliddal, H. & Danneskiold-Samsøe, B., jun. 2004, I : Gait & posture. 19, 3, s. 288-97 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The purpose of this study was to determine the test-retest reliability of a trunk accelerometric gait analysis in healthy subjects. Accelerations were measured during walking using a triaxial accelerometer mounted on the lumbar spine of the subjects. Six men and 14 women (mean age 35.2; range 18-57) underwent the same protocol on 2 consecutive days. The raw acceleration signals from six self-selected walking speeds were transformed into a horizontal-vertical coordinate system to remove unwanted variability caused by gravity. Acceleration root mean square values, cadences, step and stride lengths were then computed and interpolated using quadratic curve fits and point estimates were calculated at a standardised walking speed of 1.35 m/s. Relative reliability was determined using two models of intraclass correlation coefficients (ICC(1,1) and ICC(3,1)) to assess any systematic shifts and absolute reliability was determined using measurement error (ME). The results of the study showed high ICC values (0.77-0.96) and ME values of 0.007-0.01 g for mean acceleration; 0.009 m for step lengths; 0.022 m for stride length and 1.644 step/min for cadences. In conclusion, the method was found to be reliable and may have a definite potential in clinical gait analysis.

Originalsprog Engelsk
Tidsskrift Gait & posture
Vol/bind 19
Udgave nummer 3
Sider (fra-til) 288-97
Antal sider 10
ISSN 0966-6362
DOI
Status Udgivet - jun. 2004

Vitamin D insufficiency in Greenlanders on a westernized fare: ethnic differences in calcitropic hormones between Greenlanders and Danes

Rejnmark, L., Hansen, J. C., Heickendorff, L., Lauridsen, A. L., Mulvad, G., Siggaard, C., Skjoldborg, H., Sørensen, T. B., Pedersen, E. B., Mosekilde, L. & Jørgensen, M. E., mar. 2004, I : Calcified Tissue International. 74, 3, s. 255-63 9 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Greenlanders living in Nuuk on a westernized fare (group B), 54 Greenlanders (group C), and 43 Danes (Group D) living in Denmark (55 degrees N) on a westernized fare. Blood specimens were drawn both summer and winter. Vitamin D insufficiency (plasma 25 OHD <40 nmol/l) was common in all four study groups during summer (23-74%) and winter (42-81%). Compared to groups A and D, vitamin D insufficiency was significantly more frequent in groups B and C. In all groups, summer levels of 25 OHD were above winter levels. Multiple regression analysis revealed a significant effect of ethnicity. Compared to Danes, Greenlanders had higher 1,25-dihydroxyvitamin D levels, but lower 25 OHD and PTH levels despite relatively low plasma calcium concentrations. In addition to ethnicity, 25(OH)D levels were influenced by age, season (summer > winter), and diet (a traditional Inuit diet>westernized diet). Ethnic differences exist between Greenlanders and Danes. Our results suggest that Greenlanders may have an inherent lower "set-point" for calcium-regulated PTH release or an enhanced renal 1,25(OH)(2)D production. In addition to ethnicity, age, season, and diet were important determinants of vitamin D status. Changes from a traditional to a westernized fare are associated with a reduced vitamin D status in Greenlanders. Vitamin D supplementation should be considered.

Originalsprog Engelsk
Tidsskrift Calcified Tissue International
Vol/bind 74
Udgave nummer 3
Sider (fra-til) 255-63
Antal sider 9
ISSN 0171-967X
DOI
Status Udgivet - mar. 2004

Published in 2003

Affective disorders in neurological diseases: a case register-based study

Nilsson, F. M., Kessing, L. V., Sørensen, T. M., Andersen, P. K. & Bolwig, T. G., jul. 2003, I : Acta Psychiatrica Scandinavica. 108, 1, s. 41-50 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVE: To investigate the temporal relationships between a range of neurological diseases and affective disorders.

METHOD: Data derived from linkage of the Danish Psychiatric Central Register and the Danish National Hospital Register. Seven cohorts with neurological index diagnoses and two control group diagnoses were followed for up to 21 years. The incidences of affective disorders in the different groups were compared with the control groups, using competing risks to consider the risk of affective disorder and the risk of death in the same analysis.

RESULTS: We found an increased incidence of affective disorders in dementia, Parkinson's disease, epilepsy, stroke and intracerebral haemorrhage compared with control groups. The association was found to be the strongest for dementia and Parkinson's disease. In hospitalized patients, with incident multiple sclerosis, the incidence of affective disorder was lower than the incidence in the control groups.

CONCLUSION: In neurological diseases there seems to be an increased incidence of affective disorders. The elevated incidence was found to be particularly high for dementia and Parkinson's disease (neurodegenerative diseases).

Originalsprog Engelsk
Tidsskrift Acta Psychiatrica Scandinavica
Vol/bind 108
Udgave nummer 1
Sider (fra-til) 41-50
Antal sider 10
ISSN 0001-690X
Status Udgivet - jul. 2003

TNF-alpha-blokker-behandling af artrit--fire års opfølgning

Petri, A., Dreyer, L., Danneskiold-Samsøe, B., Terslev, L. & Bliddal, H., 24 nov. 2003, I : Ugeskrift for Laeger. 165, 48, s. 4649-52 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Bidragets oversatte titel Anti-TNF-alpha-treatment of arthritis--4-year follow-up
Originalsprog Dansk
Tidsskrift Ugeskrift for Laeger
Vol/bind 165
Udgave nummer 48
Sider (fra-til) 4649-52
Antal sider 4
ISSN 0041-5782
Status Udgivet - 24 nov. 2003

Published in 2002

Admission body temperature predicts long-term mortality after acute stroke: the Copenhagen Stroke Study

Kammersgaard, L. P., Jørgensen, H. S., Rungby, J., Reith, J., Nakayama, H., Weber, U. J., Houth, J. & Olsen, T. S. jul 2002 I : Stroke. 33, 7, s. 1759-62 4 s.

Publikation: Forskning - peer reviewTidsskriftartikel

Body temperature is considered crucial in the management of acute stroke patients. Recently hypothermia applied as a therapy for stroke has been demonstrated to be feasible and safe in acute stroke patients. In the present study, we investigated the predictive role of admission body temperature to the long-term mortality in stroke patients.
Originalsprog Engelsk
Tidsskrift Stroke
Udgivelsesdato jul 2002
Vol/bind 33
Tidsskriftsnummer 7
Sider 1759-62
Antal sider 4
ISSN 0039-2499
Status Udgivet

OBJECTIVE: To investigate the time relation between dementia and major affective disorders (major depression and mania).

METHODS: Register linkage study of the Danish Hospital Register and the Danish Psychiatric Central Research Register, to establish study cohorts of patients with dementia and control groups (osteoarthritis or diabetes) on first discharge from hospital. Follow up of cohorts was for up to 21 years. Hazard of death was allowed for by the use of competing risks models.

RESULTS: Patients with dementia had an increased risk of being admitted to hospital for major depression or mania during the course of the illness. The incidence remained elevated throughout the rest of the patient's life.

CONCLUSIONS: Patients with dementia have an increased risk of developing depression or mania. Proper treatment of affective disorders in patients with dementia is important in reducing suffering and costs.

Originalsprog Engelsk
Tidsskrift Journal of neurology, neurosurgery, and psychiatry
Vol/bind 73
Udgave nummer 1
Sider (fra-til) 40-4
Antal sider 5
ISSN 0022-3050
Status Udgivet - jul. 2002

Published in 2001

Early infection and prognosis after acute stroke: the Copenhagen Stroke Study

Kammersgaard, L. P., Jørgensen, H. S., Reith, J., Nakayama, H., Houth, J. G., Weber, U. J., Pedersen, P. M. & Olsen, T. S. 2001 I : Journal of Stroke & Cerebrovascular Diseases. 10, 5, s. 217-221 5 s.

Publikation: Forskning - peer reviewTidsskriftartikel

Infection is a frequent complication in the early course of acute stroke and may adversely affect stroke outcome. In the present study, we investigate early infection developing in patients within 3 days of admission to the hospital and its independent relation to recovery and stroke outcome. In addition, we identify predictors for early infections, infection subtypes, and their relation to initial stroke severity.
Originalsprog Engelsk
Tidsskrift Journal of Stroke & Cerebrovascular Diseases
Udgivelsesdato 2001
Vol/bind 10
Tidsskriftsnummer 5
Sider 217-221
Antal sider 5
ISSN 1052-3057
DOI
Status Udgivet

Effects of a Brussels sprouts extract on oxidative DNA damage and metabolising enzymes in rat liver

Sørensen, M., Jensen, B. R., Poulsen, H. E., Deng, X., Tygstrup, N., Dalhoff, K. & Loft, S., jun. 2001, I : Food and Chemical Toxicology. 39, 6, s. 533-40 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The apparent anticarcinogenic effect of cruciferous vegetables found in numerous epidemiological and experimental studies has been associated with their influence on phase I and phase II metabolising enzymes as well as on the antioxidant status. In the present study we investigated the effect of administration of a Brussels sprouts extract on the expression at the mRNA level and/or catalytic activity in rat liver of three phase I enzymes [cytochrome P450-1A2 (CYP1A2),-2B1/2 (CYP2B1/2) and-2E1 (CYP2E1)] and two phase II enzyme [NADPH:quinone reductase (QR) and glutathione S-transferase pi 7 (GSTpi)], all previously suggested to be induced by vegetables. We also examined the activity and/or expression of several important antioxidant enzymes: glutathione peroxidase (GPx), catalase and gamma-glutamyl-cysteine synthetase (GCS) and the activity of the repair enzyme 8-oxoguanine DNA glycosylase (OGG1). QR, GPx and catalase activity was also assessed in the kidneys. In order to examine a possible effect of the Brussels sprouts related to oxidative stress, we measured oxidative DNA damage in terms of 7-hydro-8-oxo-2'-deoxyguanosine (8-oxodG) and lipid peroxidation in terms of malondialdehyde (MDA) formation in the liver. Oral administration of an aqueous Brussels sprouts extract for 4 days was found to induce the expression of GST 1.3-fold (P < 0.05) and the activity of QR 2.6-fold in rat liver (P < 0.05). No significant differences were seen in the expression of the phase I enzymes. No differences in antioxidant enzyme activity/expression or OGG1 activity were observed. In a second experiment, administration of the Brussels sprouts extract for 3 or 7 days was found to increase the level of 8-oxodG in rat liver from 0.75 to 0.97 per 10(5) dG and from 0.81 to 0.97 per 10(5) dG, respectively (P < 0.05). No effects on MDA levels were found. The present results support the data obtained in several studies that consumption of cruciferous vegetables is capable of inducing various phase II enzyme systems. However, the observed increase in oxidative DNA damage raises the question of whether greatly increased ingestion of cruciferous vegetables is beneficial.

Originalsprog Engelsk
Tidsskrift Food and Chemical Toxicology
Vol/bind 39
Udgave nummer 6
Sider (fra-til) 533-40
Antal sider 8
ISSN 0278-6915
Status Udgivet - jun. 2001

Manual and oral apraxia in acute stroke, frequency and influence on functional outcome: The Copenhagen Stroke Study

Pedersen, P. M., Jørgensen, H. S., Kammersgaard, L. P., Nakayama, H., Raaschou, H. O. & Olsen, T. S., sep. 2001, I : American Journal of Physical Medicine and Rehabilitation. 80, 9, s. 685-92 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

To determine the frequency of manual and oral apraxia in acute stroke and to examine the influence of these symptoms on functional outcome.
Originalsprog Engelsk
Tidsskrift American Journal of Physical Medicine and Rehabilitation
Vol/bind 80
Udgave nummer 9
Sider (fra-til) 685-92
Antal sider 8
ISSN 0894-9115
Status Udgivet - sep. 2001

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