Published in 2007

Reliability and normative values of the foot line test: a technique to assess foot posture

Brushøj, C., Larsen, K., Nielsen, MB. & Hölmich, P. 2007 I : Journal of Orthopaedic and Sports Physical Therapy. 37, 11, s. 703-7

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

STUDY DESIGN: Test-retest reliability. OBJECTIVE: To examine the reliability and report normative values of a novel test, the foot line test (FLT), to describe foot morphology. BACKGROUND: Numerous foot examinations are performed each day, but most existing examination techniques have considerable limitations regarding reliability and validity. METHODS: One hundred thirty subjects with mean foot size 44 (41-50 European size) participated. Two examiners, blinded to each other's measurements, measured the right foot of the subjects twice and the left foot once. The position of the most medial aspect of the navicular in the mediolateral direction was projected vertically onto a piece of paper placed under the subject's foot, and compared to the position of the forefoot and hindfoot to obtain the FLT value. RESULTS: FLT values ranged from -8 to 14 mm, with a mean (+/-SD) of 3.7 +/- 3.4 mm. The intratester reproducibility reported by SEM was 0.8 mm for tester 1 and 0.9 mm for tester 2, while intertester SEM was 1.4 mm for the right foot and 1.3 mm for the left foot. The intrarater ICC was 0.95 for tester 1 and 0.94 for tester 2, while the interrater ICC was 0.86 (left foot) and 0.83 (right foot). There was no significant association between foot size and FLT values. CONCLUSION: The FLT is a reproducible technique to assess foot posture.
Originalsprog Engelsk
Tidsskrift Journal of Orthopaedic and Sports Physical Therapy
Vol/bind 37
Tidsskriftsnummer 11
Sider (fra-til) 703-7
ISSN 0190-6011
Status Udgivet - 2007
To compare the efficacy of adalimumab, etanercept, and infliximab in patients with established rheumatoid arthritis (RA) taking concomitant methotrexate (MTX) by calculating the number needed to treat (NNT) using three different methods.
Originalsprog Engelsk
Tidsskrift Scandinavian Journal of Rheumatology
Vol/bind 36
Tidsskriftsnummer 6
Sider (fra-til) 411-7
Antal sider 7
ISSN 0300-9742
DOI
Status Udgivet - 2007

UNLABELLED: The sensitivity of ultrasound Doppler has now reached a level at which perfusion can be detected even in normal, resting musculoskeletal tissues. To be able to distinguish normal from abnormal flow, the resistive index (RI) determined by spectral Doppler may be of value. Inflammation is associated with low RI values and a value of 1.00 is normal.

PURPOSE: In tissues with no detectable flow, it has been assumed that RI may be defined as 1.00. This study was performed to test if normal tendinous vessels could be visualised with a contrast agent, and if such vessels had normal RI.

MATERIALS AND METHODS: Ultrasound (US) was performed with a 14 MHz linear transducer. 22 asymptomatic tendons in 12 subjects were scanned, and only 5 ultrasonically normal tendons in 5 subjects were identified. In these 5 normal tendons, SonoVue was used to make vessels visible. The Achilles tendons and the peritendinous tissues were evaluated clinically.

RESULTS: In all 5 ultrasonically normal tendons, arteries could be detected with Doppler after contrast injection and in all cases with normal RI values. All vessels were located in the mid-portion of the tendon. 18 tendons were categorised as normal by the clinician.

CONCLUSION: After administration of a contrast agent, all Achilles tendons could be demonstrated to have arteries present in the mid-portion. The flow profile of these arteries was fully normal without diastolic component and with an RI of 1.00. This finding has implications for criteria of normality of tendons, which cannot be based exclusively on the presence or absence of Doppler activity. The majority of the tendons in this study had ultrasound abnormalities both on gray-scale and colour Doppler. It can be speculated that the abnormalities may be age-related degenerative changes and that age-stratified normal materials are needed to define normality. There was no consistency between US and clinical diagnosis.

Originalsprog Engelsk
Tidsskrift Ultraschall in der Medizin (Stuttgart, Germany : 1980)
Vol/bind 28
Tidsskriftsnummer 1
Sider (fra-til) 52-6
Antal sider 5
ISSN 0172-4614
DOI
Status Udgivet - feb. 2007

Published in 2006

Barthel indeks til vurdering af funktionsevne: Dansk konsensus om brug

Maribo, T., Lauritsen, J., Waehrens, E., Poulsen, I. & Hesselbo, B. 2006 I : Ugeskrift for Laeger. 168, 34, s. 2790-2 3 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The Barthel Index is widely used in the evaluation of function. In Denmark at least three versions are used without a consensus of their specific formulation. The term ''Modified Barthel Index'' is used for several versions. A Barthel Index score without version has no meaning since a maximum score in one version indicates very poor function in another. This paper describes the proper usage of the Barthel Index and suggests the terminology ''Barthel-20'' and ''Barthel-100'' for the recommended versions published by Collin et al and Shah et al. The number after ''Barthel-'' indicates the maximum score.
Originalsprog Dansk
Tidsskrift Ugeskrift for Laeger
Vol/bind 168
Tidsskriftsnummer 34
Sider (fra-til) 2790-2
Antal sider 3
ISSN 0041-5782
Status Udgivet - 2006

Degradation of the organic phase of bone by osteoclasts: a secondary role for lysosomal acidification

Henriksen, K., Sørensen, M. G., Nielsen, R. H., Gram, J., Schaller, S., Dziegiel, M. H., Everts, V., Bollerslev, J. & Karsdal, M. A. jan. 2006 I : Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research. 21, 1, s. 58-66 9 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

UNLABELLED: Osteoclasts degrade bone matrix by secretion of hydrochloric acid and proteases. We studied the processes involved in the degradation of the organic matrix of bone in detail and found that lysosomal acidification is involved in this process and that MMPs are capable of degrading the organic matrix in the absence of cathepsin K.

INTRODUCTION: Osteoclasts resorb bone by secretion of acid by the vacuolar H+-adenosine triphosphatase (V-ATPase) and the chloride channel ClC-7, followed by degradation of the matrix, mainly collagen type I, by cathepsin K and possibly by matrix metalloproteinases (MMPs). However, the switch from acidification to proteolysis and the exact roles of both the ion transporters and the proteinases still remain to be studied.

MATERIALS AND METHODS: We isolated CD14+ monocytes from human peripheral blood from either controls or patients with autosomal dominant osteopetrosis type II (ADOII) caused by defective ClC-7 function and cultured them in the presence of RANKL and macrophage-colony stimulating factor (M-CSF) to generate osteoclasts. We decalcified cortical bovine bone slices and studied the osteoclasts with respect to morphology, markers, and degradation of the decalcified matrix in the presence of various inhibitors of osteoclast acidification and proteolysis, using normal calcified bone as a reference.

RESULTS: We found that ADOII osteoclasts not only have reduced resorption of the calcified matrix, but also 40% reduced degradation of the organic phase of bone. We found that both acidification inhibitors and cathepsin K inhibitors reduced degradation of the organic matrix by 40% in normal osteoclasts, but had no effect in the ADOII osteoclasts. Furthermore, we showed that inhibition of MMPs leads to a 70% reduction in the degradation of the organic bone matrix and that MMPs and cathepsin K have additive effects. Finally, we show that osteoclastic MMPs mediate release of the carboxyterminal telopeptide of type I collagen (ICTP) fragment in the absence of cathepsin K activity, and therefore, to some extent, are able to compensate for the loss of cathepsin K activity.

CONCLUSIONS: These data clearly show that osteoclastic acidification of the lysosomes plays a hitherto nonrecognized role in degradation of the organic matrix. Furthermore, these data shed light on the complicated interplay between acidification dependent and independent proteolytic processes, mediated by cathepsin K and the MMPs, respectively.

Originalsprog Engelsk
Tidsskrift Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research
Vol/bind 21
Tidsskriftsnummer 1
Sider (fra-til) 58-66
Antal sider 9
ISSN 0884-0431
DOI
Status Udgivet - jan. 2006

Estrogen directly attenuates human osteoclastogenesis, but has no effect on resorption by mature osteoclasts

Sørensen, M. G., Henriksen, K., Dziegiel, M. H., Tankó, L. B. & Karsdal, M. A. aug. 2006 I : D N A and Cell Biology. 25, 8, s. 475-83 9 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Estrogen deficiency arising with the menopause promotes marked acceleration of bone resorption, which can be restored by hormone replacement therapy. The inhibitory effects of estrogen seem to involve indirect cytokine- mediated effects via supporting bone marrow cells, but direct estrogen-receptor mediated effects on the bone-resorbing osteoclasts have also been proposed. Little information is available on whether estrogens modulate human osteoclastogenesis or merely inhibit the functional activity of osteoclasts. To clarify whether estrogens directly modulate osteoclastic activities human CD14+ monocytes were cultured in the presence of M-CSF and RANKL to induce osteoclast differentiation. Addition of 0.1-10 nM 17beta-estradiol to differentiating osteoclasts resulted in a dose-dependent reduction in tartrate resistant acid phosphatase (TRACP) activity reaching 60% at 0.1 nM. In addition, 17beta-estradiol inhibited bone resorption, as measured by the release of the C-terminal crosslinked telopeptide (CTX), by 60% at 0.1 nM, but had no effect on the overall cell viability. In contrast to the results obtained with differentiating osteoclasts, addition of 17beta-estradiol (0.001-10 nM) to mature osteoclasts did not affect bone resorption or TRACP activity. We investigated expression of the estrogen receptors, using immunocytochemistry and Western blotting. We found that ER-alpha is expressed in osteoclast precursors, whereas ER- beta is expressed at all stages, indicating that the inhibitory effect of estrogen on osteoclastogenesis is mediated by ER-alpha for the major part. In conclusion, these results suggest that the in vivo effects of estrogen are mediated by reduction of osteoclastogenesis rather than direct inhibition of the resorptive activity of mature osteoclasts.

Originalsprog Engelsk
Tidsskrift D N A and Cell Biology
Vol/bind 25
Tidsskriftsnummer 8
Sider (fra-til) 475-83
Antal sider 9
ISSN 1044-5498
DOI
Status Udgivet - aug. 2006

Impulse-forces during walking are not increased in patients with knee osteoarthritis

Henriksen, M., Simonsen, E. B., Graven-Nielsen, T., Lund, H., Danneskiold-Samsøe, B. & Bliddal, H. aug. 2006 I : Acta Orthopaedica (Print Edition). 77, 4, s. 650-6 7 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

BACKGROUND: Impulsive forces in the knee joint have been suspected to be a co-factor in the development and progression of knee osteoarthritis. We thus evaluated the impulsive sagittal ground reaction forces (iGRF), shock waves and lower extremity joint kinematics at heel strike during walking in knee osteoarthritis (OA) patients and compared them to those in healthy subjects.

SUBJECTS AND METHODS: We studied 9 OA patients and 10 healthy subjects using three-dimensional gait analyses concentrated on the heel strike. Impulse GRF (iGRF) was measured together with peak accelerations (PA) at the tibial tuberosity and sacrum. Sagittal lower extremity joint angles at heel strike were extracted from the gait analyses. As OA is painful and pain might alter movement strategies, the patient group was also evaluated following pain relief by intraarticular lidocaine injections.

RESULTS: The two groups showed similar iGRF, similar tibial and sacral PA, and similar joint angles at heel strike. Following pain relief, the OA patients struck the ground with more extended hip and knee joints and lower tibial PA compared to the painful condition. Although such changes occurred after pain relief, all parameters were within their normal ranges.

INTERPRETATION: OA patients and healthy subjects show similar impulse-forces and joint kinematics at heel strike. Following pain relief in the patient group, changes in tibial PA and in hip and knee joint angles were observed but these were still within the normal range. Our findings make us question the hypothesis that impulse-forces generated at heel strike during walking contribute to progression of OA.

Originalsprog Engelsk
Tidsskrift Acta Orthopaedica (Print Edition)
Vol/bind 77
Tidsskriftsnummer 4
Sider (fra-til) 650-6
Antal sider 7
ISSN 1745-3674
DOI
Status Udgivet - aug. 2006

Increased joint loads during walking--a consequence of pain relief in knee osteoarthritis

Henriksen, M., Simonsen, E. B., Alkjaer, T., Lund, H., Graven-Nielsen, T., Danneskiold-Samsøe, B. & Bliddal, H. dec. 2006 I : The Knee. 13, 6, s. 445-50 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Joint pain is a primary symptom in knee osteoarthritis (OA), but the effect of pain and pain relief on the knee joint mechanics of walking is not clear. In this study, the effects of local knee joint analgesia on knee joint loads during walking were studied in a group of knee osteoarthritis patients. A group of healthy subjects was included as a reference group. The joint loads were calculated from standard gait analysis data obtained with standardised walking speed (4 km/h). The gait analyses were performed before and after pain relief by intra-articular injections of 10 mL lidocaine (1%). Pre-injection measurements revealed lower joint loads in the OA group compared to the reference group. Following injections pain during walking decreased significantly and the joint loads increased in the OA group during the late single support phase to a level comparable to the reference group. Although the patients walked with less compressive knee joint forces compared to the reference group, the effects of pain relief may accelerate the degenerative changes.

Originalsprog Engelsk
Tidsskrift The Knee
Vol/bind 13
Tidsskriftsnummer 6
Sider (fra-til) 445-50
Antal sider 6
ISSN 0968-0160
DOI
Status Udgivet - dec. 2006

Reproducibility of ultrasound and magnetic resonance imaging measurements of tendon size

Brushøj, C., Henriksen, B. M., Albrecht-Beste, E., Hölmich, P., Larsen, K. & Bachmann Nielsen, M. nov. 2006 I : Acta Radiologica. 47, 9, s. 954-9 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

PURPOSE: To investigate the intra- and inter-tester reproducibility of measurements of the Achilles tendon, tibialis anterior tendon, and the tibialis posterior tendon in football players using ultrasound (US) and magnetic resonance imaging (MRI).

MATERIAL AND METHODS: Eleven asymptomatic football players were examined. Using a standardized US scanning protocol, the tendons were examined by two observers with US for thickness, width, and cross-sectional area. One observer conducted the procedure twice. The subjects also underwent an MRI examination, and the assessment of tendon size was conducted twice by two observers.

RESULTS: The best reproducibility judged by coefficient of variation (CV) and 95% confidence interval was determined for the Achilles tendon on both US and MRI. The variability of US on measurements on the tibialis anterior and tibialis posterior tendons was less than that when using MRI. In 12 out of 18 measurements, there were systematic differences between observers as judged by one-sided F-test.

CONCLUSION: The reproducibility of the three tendons was limited. Precaution should be taken when looking for minor quantitative changes, i.e., training-induced hypertrophy, and when doing so, the Achilles tendon should be used.

Originalsprog Engelsk
Tidsskrift Acta Radiologica
Vol/bind 47
Tidsskriftsnummer 9
Sider (fra-til) 954-9
Antal sider 6
ISSN 0284-1851
DOI
Status Udgivet - nov. 2006

Ultrasound findings in adductor related groin pain

Hölmich, P. & Bachmann Nielsen, M. dec. 2006 I : Ultraschall in der Medizin (Stuttgart, Germany : 1980). 27, 6, s. 509-11 3 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Originalsprog Engelsk
Tidsskrift Ultraschall in der Medizin (Stuttgart, Germany : 1980)
Vol/bind 27
Tidsskriftsnummer 6
Sider (fra-til) 509-11
Antal sider 3
ISSN 0172-4614
DOI
Status Udgivet - dec. 2006

BACKGROUND: High resolution colour Doppler ultrasound shows intratendinous Doppler activity in patients with chronic Achilles tendinopathy. Treatment of this neovascularisation with sclerosing therapy seems to relieve the pain. However, the procedure often has to be repeated.

OBJECTIVE: To investigate the effect of electrocoagulation of the neovessels on tendon pain and tendon vascularity in patients with chronic Achilles tendinopathy.

METHODS: Colour Doppler ultrasound guided electrocoagulation was used on vessels in the ventral portion of the Achilles tendon in 11 patients (seven men, four women, mean age 41 years) with painful chronic mid-portion Achilles tendinosis. A unipolar coagulation device was used.

RESULTS: One patient dropped out after two months (dissatisfied with the results). The remaining 10 patients (91%) were satisfied. These 10 patients were still satisfied at six months of follow up and had returned to their previous level of activity. All 10 patients were "cured" after one treatment. The patient who dropped out received two treatments because of lack of progress. There was significantly reduced pain (Likert pain scale, 0-10) during activity, from a median of 7 (range 4 to 10) at baseline to 0 (0 to 8) at six months' follow up (p<0.005); and at rest, from 1.5 (1 to 5) to 0 (0 to 8) (p = 0.005). In all patients, vascularisation was unchanged at the six months follow up, with no significant change in semiquantitative or quantitative colour scoring.

CONCLUSIONS: Coagulation in the area with vessels entering the tendon appears to be effective treatment for painful chronic mid-tendinous Achilles tendinopathy. No effect on the intratendinous Doppler activity could be detected, suggesting that the effect is independent of changes in blood flow. Localisation of hyperaemia appears to be the key to the pathology and for targeting the treatment. One explanation could be that the effect is obtained by destruction of nerves accompanying the vessels.

Originalsprog Engelsk
Tidsskrift British Journal of Sports Medicine
Vol/bind 40
Tidsskriftsnummer 9
Sider (fra-til) 761-6
Antal sider 6
ISSN 0306-3674
DOI
Status Udgivet - sep. 2006

Published in 2005

Acidification of the osteoclastic resorption compartment provides insight into the coupling of bone formation to bone resorption

Karsdal, M. A., Henriksen, K., Sørensen, M. G., Gram, J., Schaller, S., Dziegiel, M. H., Heegaard, A-M., Christophersen, P., Martin, T., Christiansen, C. & Bollerslev, J. feb. 2005 I : American Journal of Pathology. 166, 2, s. 467-76 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Patients with defective osteoclastic acidification have increased numbers of osteoclasts, with decreased resorption, but bone formation that remains unchanged. We demonstrate that osteoclast survival is increased when acidification is impaired, and that impairment of acidification results in inhibition of bone resorption without inhibition of bone formation. We investigated the role of acidification in human osteoclastic resorption and life span in vitro using inhibitors of chloride channels (NS5818/NS3696), the proton pump (bafilomycin) and cathepsin K. We found that bafilomycin and NS5818 dose dependently inhibited acidification of the osteoclastic resorption compartment and bone resorption. Inhibition of bone resorption by inhibition of acidification, but not cathepsin K inhibition, augmented osteoclast survival, which resulted in a 150 to 300% increase in osteoclasts compared to controls. We investigated the effect of inhibition of osteoclastic acidification in vivo by using the rat ovariectomy model with twice daily oral dosing of NS3696 at 50 mg/kg for 6 weeks. We observed a 60% decrease in resorption (DPYR), increased tartrate-resistant acid phosphatase levels, and no effect on bone formation evaluated by osteocalcin. We speculate that attenuated acidification inhibits dissolution of the inorganic phase of bone and results in an increased number of nonresorbing osteoclasts that are responsible for the coupling to normal bone formation. Thus, we suggest that acidification is essential for normal bone remodeling and that attenuated acidification leads to uncoupling with decreased bone resorption and unaffected bone formation.

Originalsprog Engelsk
Tidsskrift American Journal of Pathology
Vol/bind 166
Tidsskriftsnummer 2
Sider (fra-til) 467-76
Antal sider 10
ISSN 0002-9440
DOI
Status Udgivet - feb. 2005

Evidence based prevention of hamstring injuries in sport

Petersen, J. & Hölmich, P. jun. 2005 I : British Journal of Sports Medicine. 39, 6, s. 319-23 5 s.

Publikation: Bidrag til tidsskriftReviewForskningpeer review

A common soft tissue injury in sports involving sprinting and jumping is the hamstring strain. A major problem with hamstring strains is the high incidence of reinjury. Muscle injuries can be classified as direct or indirect and are typically grouped into three categories according to severity. A number of potential risk factors have been proposed for hamstring strains. Only a few are evidence based and some are mainly based on theoretical assumptions. There is a lack of clinical research on the effectiveness of rehabilitation programmes for hamstring strains. Although the initial treatment of rest, ice, compression, and elevation is accepted for muscle strains, no consensus exists for their rehabilitation. Not much evidence based research has been carried out on prevention of hamstring strain. To our knowledge only two prospective studies have so far been published. As the injuries are common in football and other sports involving sprinting and jumping, there is a need for further research preferably in the form of randomised controlled trials.

Originalsprog Engelsk
Tidsskrift British Journal of Sports Medicine
Vol/bind 39
Tidsskriftsnummer 6
Sider (fra-til) 319-23
Antal sider 5
ISSN 0306-3674
DOI
Status Udgivet - jun. 2005

Learning effect of isokinetic measurements in healthy subjects, and reliability and comparability of Biodex and Lido dynamometers

Lund, H., Søndergaard, K., Zachariassen, T., Christensen, R., Bülow, P., Henriksen, M., Bartels, E. M., Danneskiold-Samsøe, B. & Bliddal, H. mar. 2005 I : Clinical physiology and functional imaging. 25, 2, s. 75-82 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The aim of this study was to examine the learning effect during a set of isokinetic measurements, to evaluate the reliability of the Biodex System 3 PRO dynamometer, and to compare the Biodex System 3 PRO and the Lido Active dynamometers on both extension and flexion over the elbow and the knee at 60 degrees s(-1). Thirteen (nine women, four men) healthy participants were measured five times using the Biodex and once using the Lido dynamometer. The intervals between the first four tests were 20 min, and 1 week between tests 4 and 5. Between Biodex and Lido measurements there was a 20 min time interval. When comparing the first five measurements (Biodex), no systematic effect over time and an excellent reliability were found with respect to elbow and knee flexion and extension. No difference in muscle strength (Nm) between the Biodex and Lido was observed for knee flexion (P = 0.59), knee extension (P = 0.18) and elbow extension (P = 0.63). However, elbow flexion showed a 14.8% (95% CI: 11.2-18.4%; P = 0.0001) higher peak torque on Biodex. In conclusion, no learning effect was observed and the Biodex proved to be a highly reliable isokinetic dynamometer. A difference was observed when comparing Biodex and Lido on elbow flexion, but the difference did not outrange the expected variation found with a typical isokinetic measurement, which is why both sets of equipment seem applicable in clinical practice.

Originalsprog Engelsk
Tidsskrift Clinical physiology and functional imaging
Vol/bind 25
Tidsskriftsnummer 2
Sider (fra-til) 75-82
Antal sider 8
ISSN 1475-0961
DOI
Status Udgivet - mar. 2005

Reduction of the disintegrin and metalloprotease ADAM12 in preeclampsia

Laigaard, J., Sørensen, T., Placing, S., Holck, P., Fröhlich, C., Wøjdemann, K. R., Sundberg, K., Shalmi, A-C., Tabor, A., Nørgaard-Pedersen, B., Ottesen, B., Christiansen, M. & Wewer, U. M. jul. 2005 I : Obstetrics and Gynecology. 106, 1, s. 144-9 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVES: The secreted form of ADAM12 is a metalloprotease that may be involved in placental and fetal growth. We examined whether the concentration of ADAM12 in first-trimester maternal serum could be used as a marker for preeclampsia.

METHODS: We developed a semiautomated, time-resolved, immunofluorometric assay for the quantification of ADAM12 in serum. The assay detected ADAM12 in a range of 78-1248 microg/L. Serum samples derived from women in the first trimester of a normal pregnancy (n = 324) and from women who later developed preeclampsia during pregnancy (n = 160) were obtained from the First Trimester Copenhagen Study. ADAM12 levels were assayed in these serum samples. Serum levels of ADAM12 were converted to multiples of the median (MoM) after log-linear regression of concentration versus gestational age.

RESULTS: Serum ADAM12 levels in women who developed preeclampsia during pregnancy had a mean log MoM of -0.066, which was significantly lower than the mean log MoM of -0.001 for ADAM12 levels observed in serum samples from women with normal pregnancy (P = .008). The mean log MoM was even lower in serum derived from preeclamptic women whose infant's weight at birth was less than 2,500 g (n = 27, mean log MoM of -0.120, P = .053).

CONCLUSION: The maternal serum levels of ADAM12 are significantly lower during the first trimester in women who later develop preeclampsia during pregnancy when compared with levels in women with normal pregnancies. Because the secreted form of ADAM12 cleaves insulin-like growth factor binding protein (IGFBP)-3 and IGFBP-5, the IGF axis may play a role in preeclampsia. ADAM12 may be a useful early marker for preeclampsia.

LEVEL OF EVIDENCE: II-2.

Originalsprog Engelsk
Tidsskrift Obstetrics and Gynecology
Vol/bind 106
Tidsskriftsnummer 1
Sider (fra-til) 144-9
Antal sider 6
ISSN 0029-7844
DOI
Status Udgivet - jul. 2005

Published in 2004

Plasma TNF binding capacity profiles during treatment with etanercept in rheumatoid arthritis

Gudbrandsdottir, S., Bliddal, H., Petri, A., Terslev, L., Danneskiold-Samsøe, B., Bjørnhart, B., Bendtzen, K. & Müller, K. 1 jan. 2004 I : Scandinavian Journal of Rheumatology. 33, 6, s. 385-8 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Etanercept (Enbrel) induces a rapid and sustained decline in disease activity in the majority of patients with refractory rheumatoid arthritis (RA). In these patients neutralization of TNFalpha and lymphotoxin (LT), previously termed TNFbeta is mediated by etanercept itself, as well as by naturally occurring soluble TNF receptors. However, the clinical response to treatment with etanercept may vary. Previously, pharmacokinetic studies have focused on the molar concentrations of etanercept, but very little is known about the kinetics of bioactive etanercept in patients treated with etanercept. The purpose of this study was to evaluate kinetics, including inter- and intraindividual variations of the total TNF binding capacity, in RA patients who were on a standard treatment schedule with etanercept.
Originalsprog Engelsk
Tidsskrift Scandinavian Journal of Rheumatology
Vol/bind 33
Tidsskriftsnummer 6
Sider (fra-til) 385-8
Antal sider 4
ISSN 0300-9742
Status Udgivet - 1 jan. 2004

Test-retest reliability of trunk accelerometric gait analysis

Henriksen, M., Lund, H., Moe-Nilssen, R., Bliddal, H. & Danneskiold-Samsøe, B. jun. 2004 I : Gait & posture. 19, 3, s. 288-97 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The purpose of this study was to determine the test-retest reliability of a trunk accelerometric gait analysis in healthy subjects. Accelerations were measured during walking using a triaxial accelerometer mounted on the lumbar spine of the subjects. Six men and 14 women (mean age 35.2; range 18-57) underwent the same protocol on 2 consecutive days. The raw acceleration signals from six self-selected walking speeds were transformed into a horizontal-vertical coordinate system to remove unwanted variability caused by gravity. Acceleration root mean square values, cadences, step and stride lengths were then computed and interpolated using quadratic curve fits and point estimates were calculated at a standardised walking speed of 1.35 m/s. Relative reliability was determined using two models of intraclass correlation coefficients (ICC(1,1) and ICC(3,1)) to assess any systematic shifts and absolute reliability was determined using measurement error (ME). The results of the study showed high ICC values (0.77-0.96) and ME values of 0.007-0.01 g for mean acceleration; 0.009 m for step lengths; 0.022 m for stride length and 1.644 step/min for cadences. In conclusion, the method was found to be reliable and may have a definite potential in clinical gait analysis.

Originalsprog Engelsk
Tidsskrift Gait & posture
Vol/bind 19
Tidsskriftsnummer 3
Sider (fra-til) 288-97
Antal sider 10
ISSN 0966-6362
DOI
Status Udgivet - jun. 2004

Vitamin D insufficiency in Greenlanders on a westernized fare: ethnic differences in calcitropic hormones between Greenlanders and Danes

Rejnmark, L., Hansen, J. C., Heickendorff, L., Lauridsen, A. L., Mulvad, G., Siggaard, C., Skjoldborg, H., Sørensen, T. B., Pedersen, E. B., Mosekilde, L. & Jørgensen, M. E. mar. 2004 I : Calcified tissue international. 74, 3, s. 255-63 9 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

We studied the influence of age, gender, latitude, season, diet and ethnicity on plasma 25-hydroxyvitamin D 25 OHD, PTH, 1,25-dihydroxyvitamin D, vitamin D-binding protein, bone-specific alkaline phosphatase, and osteocalcin levels in 46 Greenlanders living in Nuuk (64 degrees N) on a traditional fare (group A), 45 Greenlanders living in Nuuk on a westernized fare (group B), 54 Greenlanders (group C), and 43 Danes (Group D) living in Denmark (55 degrees N) on a westernized fare. Blood specimens were drawn both summer and winter. Vitamin D insufficiency (plasma 25 OHD <40 nmol/l) was common in all four study groups during summer (23-74%) and winter (42-81%). Compared to groups A and D, vitamin D insufficiency was significantly more frequent in groups B and C. In all groups, summer levels of 25 OHD were above winter levels. Multiple regression analysis revealed a significant effect of ethnicity. Compared to Danes, Greenlanders had higher 1,25-dihydroxyvitamin D levels, but lower 25 OHD and PTH levels despite relatively low plasma calcium concentrations. In addition to ethnicity, 25(OH)D levels were influenced by age, season (summer > winter), and diet (a traditional Inuit diet>westernized diet). Ethnic differences exist between Greenlanders and Danes. Our results suggest that Greenlanders may have an inherent lower "set-point" for calcium-regulated PTH release or an enhanced renal 1,25(OH)(2)D production. In addition to ethnicity, age, season, and diet were important determinants of vitamin D status. Changes from a traditional to a westernized fare are associated with a reduced vitamin D status in Greenlanders. Vitamin D supplementation should be considered.

Originalsprog Engelsk
Tidsskrift Calcified tissue international
Vol/bind 74
Tidsskriftsnummer 3
Sider (fra-til) 255-63
Antal sider 9
ISSN 0171-967X
DOI
Status Udgivet - mar. 2004

Published in 2003

Affective disorders in neurological diseases: a case register-based study

Nilsson, F. M., Kessing, L. V., Sørensen, T. M., Andersen, P. K. & Bolwig, T. G. jul. 2003 I : Acta Psychiatrica Scandinavica. 108, 1, s. 41-50 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVE: To investigate the temporal relationships between a range of neurological diseases and affective disorders.

METHOD: Data derived from linkage of the Danish Psychiatric Central Register and the Danish National Hospital Register. Seven cohorts with neurological index diagnoses and two control group diagnoses were followed for up to 21 years. The incidences of affective disorders in the different groups were compared with the control groups, using competing risks to consider the risk of affective disorder and the risk of death in the same analysis.

RESULTS: We found an increased incidence of affective disorders in dementia, Parkinson's disease, epilepsy, stroke and intracerebral haemorrhage compared with control groups. The association was found to be the strongest for dementia and Parkinson's disease. In hospitalized patients, with incident multiple sclerosis, the incidence of affective disorder was lower than the incidence in the control groups.

CONCLUSION: In neurological diseases there seems to be an increased incidence of affective disorders. The elevated incidence was found to be particularly high for dementia and Parkinson's disease (neurodegenerative diseases).

Originalsprog Engelsk
Tidsskrift Acta Psychiatrica Scandinavica
Vol/bind 108
Tidsskriftsnummer 1
Sider (fra-til) 41-50
Antal sider 10
ISSN 0001-690X
Status Udgivet - jul. 2003

TNF-alpha-blokker-behandling af artrit--fire års opfølgning

Petri, A., Dreyer, L., Danneskiold-Samsøe, B., Terslev, L. & Bliddal, H. 24 nov. 2003 I : Ugeskrift for laeger. 165, 48, s. 4649-52 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Originalsprog Dansk
Tidsskrift Ugeskrift for laeger
Vol/bind 165
Tidsskriftsnummer 48
Sider (fra-til) 4649-52
Antal sider 4
ISSN 0041-5782
Status Udgivet - 24 nov. 2003

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