Published in 2009

Blood glucose in acute stroke

Olsen, T. S. 2009 I : Expert review of neurotherapeutics. 9, 3, s. 409-19 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Udgivelsesdato: 2009-Mar
Originalsprog Engelsk
Tidsskrift Expert review of neurotherapeutics
Vol/bind 9
Tidsskriftsnummer 3
Sider (fra-til) 409-19
Antal sider 10
DOI
Status Udgivet - 2009

Can stimulating massage improve joint repositioning error in patients with knee osteoarthritis?

Lund, H., Henriksen, M., Bartels, E. M., Danneskiold-Samsøe, B. & Bliddal, H. 2009 I : Journal of Geriatric Physical Therapy. 32, 3, s. 111-6 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

PURPOSE: The purpose of this study was to investigate the effect of massage applied to the thigh muscles on joint repositioning error (JRE) in patients suffering from osteoarthritis (OA).We hypothesized that stimulating massage of the muscles around an osteoarthritic knee joint, could improve the neuromuscular function and thereby optimize the positive and minimize the negative performance factors in relation to an exercise program.

METHODS: In a cross-over design, 19 patients with knee osteoarthritis, mean age of 73.1 years (SD: 9.4; range 56 to 88 years), recruited from the local department of rheumatology, were randomly allocated to either receive massage and a week later, act as controls or vice versa. The applied massage consisted of stimulating massage of the quadriceps femoris, sartorious, gracilus, and hamstrings muscles for 10 min on the affected leg. Participants had their JRE measured before and immediately after the 10 min massage and control sessions. Data were analyzed by using paired t-test.

RESULTS: No significant change in JRE was observed (95% CI: -0.62 degrees to 0.85 degrees, p = 0.738).

CONCLUSION: Massage has no effect on the immediate joint repositioning error in patients with knee osteoarthritis, ie, if an improved JRE is important for improving the performance factors when exercising, stimulating massage may not be recommendable to use.

Originalsprog Engelsk
Tidsskrift Journal of Geriatric Physical Therapy
Vol/bind 32
Tidsskriftsnummer 3
Sider (fra-til) 111-6
Antal sider 6
ISSN 1539-8412
Status Udgivet - 2009

Disease-modifying anti-rheumatic drugs til behandling af ankyloserende spondylitis

Madsen, O. R. & Egsmose, C. 2009 I : Ugeskrift for Laeger. 171, 33, s. 2268-72 5 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Ankylosing spondylitis (AS) is an inflammatory disorder affecting the axial skeleton, peripheral joints, entheses and extra-articular sites. Patients with early disease, a higher level of erythrocyte sedimentation rate and/or peripheral arthritis might benefit from sulfasalazine. Otherwise, there is no evidence that disease-modifying anti-rheumatic (DMARDs) have a therapeutic effect in AS. Clinical evidence that greater TNF-inhibitor effectiveness can be achieved by combining with a DMARD is lacking, but further studies should be performed. More research is needed to clarify the role of DMARDs in the treatment of AS.
Originalsprog Dansk
Tidsskrift Ugeskrift for Laeger
Vol/bind 171
Tidsskriftsnummer 33
Sider (fra-til) 2268-72
Antal sider 5
ISSN 0041-5782
Status Udgivet - 2009

Effect of whole body vibration exercise on muscle strength and proprioception in females with knee osteoarthritis

Trans, T., Aaboe, J., Henriksen, M., Christensen, R., Bliddal, H. & Lund, H. aug. 2009 I : The Knee. 16, 4, s. 256-61 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The purpose of this study was to assess the effect of whole body vibration (WBV) exercise on muscle strength and proprioception in female patients with osteoarthritis in the knee (knee-OA). A single blinded, randomised, controlled trial was performed in an outpatient clinic on 52 female patients diagnosed with knee-OA (mean age 60.4 years+/-9.6). They were randomly assigned to one of 3 groups: 1. WBV-exercise on a stable platform (VibM; n=17 (mean age, 61.5+/-9.2)), WBV-exercise on a balance board (VibF; n=18 (mean age, 58.7+/-11.0)), or control group (Con; n=18 (mean age, 61.1+/-8.5)). The WBV groups trained twice a week for 8 weeks, with a progressively increasing intensity. The WBV groups performed unloaded static WBV exercise. The following were measured: knee muscle strength (extension/flexion) and proprioception (threshold for detection of passive movement (TDPM)). Self-reported disease status was measured using WOMAC. It was found that muscle strength increased significantly (p<0.001) in VibM compared to Con. Isometric knee-extension significantly increased (p=0.021) in VibM compared to Con. TDPM was significantly improved (p=0.033) in VibF compared to Con, while there was a tendency (p=0.051) for VibM to perform better compared to Con. There were no effects in the self-reported disease status measures. This study showed that the WBV-exercise regime on a stable platform (VibM) yielded increased muscle strength, while the WBV-exercise on a balance board (VibF) showed improved TDPM. The WBV-exercise is a time-saving and safe method for rehabilitation of women with knee-OA.

Originalsprog Engelsk
Tidsskrift The Knee
Vol/bind 16
Tidsskriftsnummer 4
Sider (fra-til) 256-61
Antal sider 6
ISSN 0968-0160
DOI
Status Udgivet - aug. 2009

Experimental muscle pain during a forward lunge--the effects on knee joint dynamics and electromyographic activity

Henriksen, M., Alkjaer, T., Simonsen, E. B. & Bliddal, H. jul. 2009 I : British Journal of Sports Medicine. 43, 7, s. 503-7 5 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVE: The purpose of this study was to investigate whether the knee joint dynamics during a forward lunge could be modulated by experimentally induced vastus medialis pain in healthy subjects.

DESIGN: Randomised cross-over study.

SETTING: Biomechanical movement laboratory.

PARTICIPANTS: 20 healthy subjects were included. One subject was excluded during data collection.

INTERVENTION: The subjects performed forward lunges before, during and 20 minutes after induction of experimental quadriceps muscle pain. Muscle pain was induced using hypertonic saline (5.8%) injected intramuscularly. Isotonic saline (0.9%) was used as control.

MAIN OUTCOME MEASUREMENTS: Three-dimensional movement analyses were performed and inverse dynamics were used to calculate joint kinematics and kinetics for ankle, knee and hip joints. Electromyographic (EMG) signals of the hamstrings and quadriceps muscles were recorded.

RESULTS: During and after pain, significant decreases in knee joint dynamics and EMG recordings were observed.

CONCLUSION: The study shows that local pain in the quadriceps is capable of modulating movements with high knee joint dynamics. The results may have implications in the management of muscle pain and prevention of injuries during activities involving the knee joint.

Originalsprog Engelsk
Tidsskrift British Journal of Sports Medicine
Vol/bind 43
Tidsskriftsnummer 7
Sider (fra-til) 503-7
Antal sider 5
ISSN 0306-3674
DOI
Status Udgivet - jul. 2009

Experimentally reduced hip abductor function during walking: Implications for knee joint loads

Henriksen, M., Aaboe, J., Simonsen, E. B., Alkjaer, T. & Bliddal, H. 19 jun. 2009 I : Journal of Biomechanics. 42, 9, s. 1236-40 5 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Hip and knee functions are intimately connected and reduced hip abductor function might play a role in development of knee osteoarthritis (OA) by increasing the external knee adduction moment during walking. The purpose of this study was to test the hypothesis that reduced function of the gluteus medius (GM) muscle would lead to increased external knee adduction moment during level walking in healthy subjects. Reduced GM muscle function was induced experimentally, by means of intramuscular injections of hypertonic saline that produced an intense short-term muscle pain and reduced muscle function. Isotonic saline injections were used as non-painful control. Fifteen healthy subjects performed walking trials at their self-selected walking speed before and immediately after injections, and again after 20 min of rest, to ensure pain recovery. Standard gait analyses were used to calculate three-dimensional trunk and lower extremity joint kinematics and kinetics. Surface electromyography (EMG) of the glutei, quadriceps, and hamstring muscles were also measured. The peak GM EMG activity had temporal concurrence with peaks in frontal plane moments at both hip and knee joints. The EMG activity in the GM muscle was significantly reduced by pain (-39.6%). All other muscles were unaffected. Peaks in the frontal plane hip and knee joint moments were significantly reduced during pain (-6.4% and -4.2%, respectively). Lateral trunk lean angles and midstance hip joint adduction and knee joint extension angles were reduced by 1 degrees . Thus, the gait changes were primarily caused by reduced GM function. Walking with impaired GM muscle function due to pain significantly reduced the external knee adduction moment. This study challenge the notion that reduced GM function due to pain would lead to increased loads at the knee joint during level walking.

Originalsprog Engelsk
Tidsskrift Journal of Biomechanics
Vol/bind 42
Tidsskriftsnummer 9
Sider (fra-til) 1236-40
Antal sider 5
ISSN 0021-9290
DOI
Status Udgivet - 19 jun. 2009

Fibromyalgi, diagnostik og praevalens. Kan kønsforskellen forklares?

Bartels, E. M., Dreyer, L., Jacobsen, S., Jespersen, A., Bliddal, H. & Danneskiold-Samsøe, B. 30 nov. 2009 I : Ugeskrift for laeger. 171, 49, s. 3588-92 5 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Most non-inflammatory musculoskeletal diseases are more common in women than in men. Fibromyalgia is characterised by chronic generalised muscle pain. The male:female ratio is 1:9. Interacting factors including genetic, hormonal, environmental and behavioural elements may cause this condition, and there are possibly subgroups of which one has shown to be treatable. A different pathogenetic appearance in the two sexes may also be present. The gender difference may partly be explained by the fact that pressure pain test in tender points forms part of the diagnosis. This may leave some male fibromyalgia patients unrecognized.
Originalsprog Dansk
Tidsskrift Ugeskrift for laeger
Vol/bind 171
Tidsskriftsnummer 49
Sider (fra-til) 3588-92
Antal sider 5
Status Udgivet - 30 nov. 2009

Forward lunge as a functional performance test in ACL deficient subjects: test-retest reliability

Alkjaer, T., Henriksen, M., Dyhre-Poulsen, P. & Simonsen, E. B. jun. 2009 I : The Knee. 16, 3, s. 176-82 7 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The forward lunge movement may be used as a functional performance test of anterior cruciate ligament (ACL) deficient and reconstructed subjects. The purposes were 1) to determine the test-retest reliability of a forward lunge in healthy subjects and 2) to determine the required numbers of repetitions necessary to yield satisfactory reliability. Nineteen healthy subjects performed four trials of a forward lunge on two different days. The movement time, impulses of the ground reaction forces (IFz, IFy), knee joint kinematics and dynamics during the forward lunge were calculated. The relative reliability was determined by calculation of Intraclass Correlation Coefficients (ICC). The IFz, IFy and the positive work of the knee extensors showed excellent reliability (ICC >0.75). All other variables demonstrated acceptable reliability (0.4>ICC<0.75). The relative reliability increased when more than a single forward lunge was used. In conclusion, the reliability was acceptable. It is recommended to represent the biomechanical variables as an average of at least three trials of the forward lunge.

Originalsprog Engelsk
Tidsskrift The Knee
Vol/bind 16
Tidsskriftsnummer 3
Sider (fra-til) 176-82
Antal sider 7
ISSN 0968-0160
DOI
Status Udgivet - jun. 2009

Hemorrhagic and ischemic strokes compared: stroke severity, mortality, and risk factors

Andersen, K. K., Olsen, T. S., Dehlendorff, C. & Kammersgaard, L. P. 2009 I : Stroke. 40, 6, s. 2068-72 4 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Udgivelsesdato: 2009-Jun
Originalsprog Engelsk
Tidsskrift Stroke
Vol/bind 40
Tidsskriftsnummer 6
Sider (fra-til) 2068-72
Antal sider 4
ISSN 0039-2499
DOI
Status Udgivet - 2009

OBJECTIVE: Use of ultrasound Doppler (USD) in diagnosing and treatment monitoring of patients with RA has increased considerably. Hyperaemia is an integral part of the inflammatory response, and the amount of USD activity in an inflamed synovium may therefore be used to quantify the inflammatory activity. It is unclear, however, whether the hyperaemia alone reflects the disease activity or may be influenced by other factors.

METHODS: Twenty-nine patients with RA underwent USD examination of the wrist before and immediately after three interventions. The interventions were carried out on three separate days. The interventions were (i) isometric exercise of the muscles of the hand and forearm, (ii) heating and (iii) cooling of the hand. The amount of Doppler in the wrist joint was quantified by measuring the percentage of colour in the synovium-the colour fraction (CF). The CF values estimated before and after each intervention were compared to see if any intervention affected the amount of Doppler in the synovium.

RESULTS: The CF decreased significantly after cooling of the hand (P = 0.018 and <0.0001). Despite being highly significant, the numerical decrease in CF was only modest, 0.78-1.33 percentage points. The other interventions did not affect the CF significantly, with P-values of 0.65 and 0.59 in the heating intervention and 0.49 in the exercise intervention.

CONCLUSIONS: Cooling of the hand should, if possible, be avoided before a USD examination of the wrist in patients with RA, because the amount of Doppler activity might be affected by low skin temperatures.

Originalsprog Engelsk
Tidsskrift Rheumatology (Online)
Vol/bind 48
Tidsskriftsnummer 12
Sider (fra-til) 1520-3
Antal sider 4
ISSN 1462-0332
DOI
Status Udgivet - dec. 2009

Proposal for levels of evidence schema for validation of a soluble biomarker reflecting damage endpoints in rheumatoid arthritis, psoriatic arthritis, and ankylosing spondylitis, and recommendations for study design

Maksymowych, W. P., Fitzgerald, O., Wells, G. A., Gladman, D. D., Landewé, R., Østergaard, M., Taylor, W. J., Christensen, R., Tak, P-P., Boers, M., Syversen, S. W., Bathon, J. M., Ritchlin, C. J., Mease, P. J., Bykerk, V. P., Garnero, P., Geusens, P., El-Gabalawy, H., Aletaha, D., Inman, R. D., Kraus, V. B., Kvien, T. K. & van der Heijde, D. aug. 2009 I : Journal of Rheumatology. 36, 8, s. 1792-9 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVE: At OMERACT 8 a framework for levels of evidence was proposed for the validation of biomarkers as surrogate outcome measures. We aimed to adapt this scheme in order to apply it in the setting of soluble biomarkers proposed to replace the measurement of damage endpoints in rheumatoid arthritis (RA), psoriatic arthritis (PsA), and ankylosing spondylitis (AS). We also aimed to generate consensus on minimum standards for the design of longitudinal studies aimed at validating biomarkers.

METHODS: Before the meeting, the Soluble Biomarker Working Group prepared a preliminary framework and discussed various models for association and prediction related to the statistical strength domain. In addition, 3 Delphi exercises addressing longitudinal study design for RA, PsA, and AS were conducted within the working group and members of the Assessments in SpondyloArthritis International Society (ASAS) and the Group for Research and Assessment of Psoriasis and Psoriatic Arthritis (GRAPPA). This formed the basis for discussions among OMERACT 9 participants.

RESULTS: The proposed framework was accepted by consensus. In the study design domain a requirement for both prospective observational studies and randomized controlled trials (RCT) in different drug classes was noted. A template for determining the level of statistical strength was proposed. The addition of a new domain on biomarker assay performance was considered essential, and participants suggested that for any biomarker this domain should be addressed first, i.e., before starting clinical validation studies. Participants agreed on most elements of a longitudinal study design template. Where consensus was lacking the working group has drafted solutions that constitute a basis for prospective validation studies.

CONCLUSION: The OMERACT 9 Soluble Biomarker Group has successfully formulated a levels of evidence scheme and a study design template that will provide guidance to conduct validation studies in the setting of soluble biomarkers proposed to replace the measurement of damage endpoints in RA, PsA, and AS.

Originalsprog Engelsk
Tidsskrift Journal of Rheumatology
Vol/bind 36
Tidsskriftsnummer 8
Sider (fra-til) 1792-9
Antal sider 8
ISSN 0315-162X
DOI
Status Udgivet - aug. 2009
To test the hypothesis that fibromyalgia (FM) patients with reduced lower extremity strength are more symptomatic and tender than FM patients with normal muscle strength.
Originalsprog Engelsk
Tidsskrift Arthritis & Rheumatism
Vol/bind 61
Tidsskriftsnummer 6
Sider (fra-til) 732-9
Antal sider 8
ISSN 0004-3591
DOI
Status Udgivet - 2009

The Bath metrology index as assessed by a trained and an untrained rater in patients with spondylarthropathy: a study of intra- and inter-rater agreements

Madsen, O. R., Hansen, L. B., Rytter, A., Suetta, C. & Egsmose, C. jan. 2009 I : Clinical rheumatology. 28, 1, s. 35-40 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The Bath ankylosing spondylitis metrology index (BASMI; range 0-10) has gained widespread use in daily clinical practice as an objective measure of spinal stiffness not only in patients with ankylosing spondylitis (AS) but also in patients with other spondylarthropathies (SpA). We examined intra-rater and inter-rater reproducibility of BASMI scoring in 30 Danish patients with SpA (median age 40 years, range 22-56 years) fulfilling the European Spondylarthropathy Study Group criteria, 25 of them satisfying the modified New York Criteria for AS. Measurements were performed twice on two different days (median interval 7 days, range 4-11) by a trained physiotherapist (PT) and by an untrained nurse who had undergone a single 1-h training session with the PT. The median BASMI score obtained by the PT on the two test days was 3.5 (range 1-8) and 3.0 (range 1-8), respectively (NS). Test-retest BASMI scores from the PT were significantly correlated (r(s) = 0.95, p < 0.0001). The 95% likely range for the difference between a patient's BASMI scores from two tests was +/-1.4 corresponding to a minimal detectable difference of +/-2 in the individual patient as the scale consists of intervals of 1. Similar results were achieved by the nurse. BASMI scores obtained by the two raters were significantly inter-correlated (r(s) = 0.95, p < 0.0001). The mean difference between paired BASMI scores obtained by the nurse and the PT on test day 1 was -0.2 with a minimal detectable difference of +/-2. A similar result was found using data from test day 2. In conclusion, a change in BASMI less than 2 may be due solely to expected random measurement error. A single 1-h training session allowed an untrained nurse to obtain BASMI results almost identical to those of an experienced PT.

Originalsprog Engelsk
Tidsskrift Clinical rheumatology
Vol/bind 28
Tidsskriftsnummer 1
Sider (fra-til) 35-40
Antal sider 6
ISSN 0770-3198
DOI
Status Udgivet - jan. 2009

Toxic hepatitis induced by infliximab in a patient with rheumatoid arthritis with no relapse after switching to etanercept

Carlsen, K. M., Riis, L. & Madsen, O. R. 1 aug. 2009 I : Clinical Rheumatology. 28, 8, s. 1001-3 3 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

We present a case of toxic hepatitis related to infliximab treatment in a 38-year-old woman with rheumatoid arthritis (RA). The patient had previously been treated with different disease-modifying drugs (DMARDs) alone or in combination but had never revealed signs of liver dysfunction. Due to high disease activity, treatment with infliximab (3 mg/kg i.v.) was initiated in combination with methotrexate (MTX) (25 mg/week) and folic acid (5 mg/week). The patient stopped MTX and folic acid on her own initiative after 3 weeks due to improvement of joint symptoms. After seven infusions, progressive elevations of the transaminases up to five times the upper normal limit were noted and treatment with infliximab was terminated. Serological tests for viral and autoimmune hepatitis and for ANA and anti-dsDNA were all negative. Specific infliximab antibodies could not be detected. Ultrasound of the liver was normal. Liver biopsy showed late signs of acute toxic hepatitis without MTX-related fibrosis. This is one the first cases that convincingly demonstrates that infliximab treatment may cause toxic hepatitis. Moreover, the case suggests a lack of hepatic cross-toxicity between infliximab and etanercept as the patient continued with etanercept without new episodes of liver dysfunction.
Originalsprog Engelsk
Tidsskrift Clinical Rheumatology
Vol/bind 28
Tidsskriftsnummer 8
Sider (fra-til) 1001-3
Antal sider 3
ISSN 0770-3198
DOI
Status Udgivet - 1 aug. 2009

Ultrasound colour Doppler measurements in a single joint as measure of disease activity in patients with rheumatoid arthritis--assessment of concurrent validity

Ellegaard, K., Torp-Pedersen, S., Terslev, L., Danneskiold-Samsøe, B., Henriksen, M. & Bliddal, H. 2009 I : Rheumatology. 48, 3, s. 254-257 3 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

OBJECTIVE: Colour Doppler ultrasound (CDU) displays blood flow in the tissues and is able to detect hyperaemia. Because hyperaemia is part of the inflammatory response, the amount of CDU activity in the inflamed synovium may be used to quantify the inflammatory activity in RA. It has never been investigated if the amount of CDU activity in a single joint can be used to quantify disease activity in RA. METHODS: A total of 109 patients with RA and affection of the wrist joint underwent a standardized CDU examination assessing three positions in their most affected wrist at start up in biological treatment. On the same day the following measures of disease activity were collected: assessment of the number of tender and swollen joints, CRP, ESR and 28-joint disease activity score (DAS28). The amount of CDU activity was quantified by measuring the percentage of colour in the synovium--the colour fraction (CF). Correlation between CF and other measures of disease activity was calculated. RESULTS: There was a significant correlation between CF and DAS28 (r = 0.29; P < 0.001), swollen joint count (r = 0.35; P < 0.001), CRP (r = 0.5; P < 0.001) and ESR (r = 0.5; P < 0.001). No other significant correlations were found. CONCLUSION: A standardized ultrasound examination of a single affected wrist joint in patients with RA may be used as a measure of disease activity. More studies are needed to identify the number of joints needed to examine by CDU to obtain the best validity of Doppler measurements
Udgivelsesdato: 2009/3
Originalsprog Engelsk
Tidsskrift Rheumatology
Vol/bind 48
Tidsskriftsnummer 3
Sider (fra-til) 254-257
Antal sider 3
ISSN 1462-0324
Status Udgivet - 2009

Published in 2008

Influence of pain and gender on impact loading during walking: a randomised trial

Henriksen, M., Christensen, R., Alkjaer, T., Lund, H., Simonsen, E. B. & Bliddal, H. feb. 2008 I : Clinical biomechanics (Bristol, Avon). 23, 2, s. 221-30 10 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

BACKGROUND: Knee joint osteoarthritis is painful and with an overweight of female incidence. The cardinal symptom is pain, which causes compensatory gait changes, and gender differences in pain sensitivity exist. Impact loadings at heel strike during walking are suspected as a co-factor in development of knee osteoarthritis. Thus the purpose of this study was to investigate the influence of experimental muscle pain and gender on generation and attenuation of impact loading during walking.

METHODS: Ten healthy males and 10 healthy females were recruited. Impact loadings during walking were measured using force platforms and accelerometers attached to the tibia and sacrum. Impact ground reaction force peaks and loading rates, and peak accelerations were used to quantify impact loadings. Attenuation was quantified by means of a transfer function between the tibial and sacral accelerometer signals, and the relative peak acceleration reduction. Knee joint kinematics were collected using a three-dimensional movement analysis system. The study was a cross-over study and data were collected before, during, and after experimental vastus medialis pain and a control situation.

FINDINGS: Experimental muscle pain did not affect generation or attenuation of impact loading in either gender. While the impact loading magnitude was similar across genders, lower loading rates and more efficient attenuation were observed in females.

INTERPRETATION: It is concluded that generation and attenuation of impact loadings during walking are independent of quadriceps pain in both genders. The present study does not provide any evidence of the tested variables to address the gender differences in loading rates and attenuation.

Originalsprog Engelsk
Tidsskrift Clinical biomechanics (Bristol, Avon)
Vol/bind 23
Tidsskriftsnummer 2
Sider (fra-til) 221-30
Antal sider 10
ISSN 0268-0033
DOI
Status Udgivet - feb. 2008

Ion transporters involved in acidification of the resorption lacuna in osteoclasts

Henriksen, K., Sørensen, M. G., Jensen, V. K., Dziegiel, M. H., Nosjean, O. & Karsdal, M. A. sep. 2008 I : Calcified tissue international. 83, 3, s. 230-42 13 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Osteoclasts possess a large amount of ion transporters, which participate in bone resorption; of these, the vacuolar-adenosine trisphosphatase (V-ATPase) and the chloride-proton antiporter ClC-7 acidify the resorption lacuna. However, whether other ion transporters participate in this process is currently not well understood. We used a battery of ion channel inhibitors, human osteoclasts, and their subcellular compartments to perform an unbiased analysis of the importance of the different ion transporters for acidification of the resorption lacuna in osteoclasts. CD14(+) monocytes from human peripheral blood were isolated, and mature osteoclasts were generated using RANKL and M-CSF. The human osteoclasts were (1) used for acridine orange assays for evaluation of lysosomal acidification, (2) used for bone resorption assays, (3) used for generation of osteoclasts membranes for acid influx experiments, or (4) lysed in trizol for mRNA isolation for Affymetrix array analysis. Inhibitors targeted toward most of the ion transporters showed low potency in the acidification-based assays, although some inhibitors, such as carbonic anhydrase II and the sodium-hydrogen exchanger (NHE) inhibitors, reduced resorption potently. In contrast, inhibitors targeted at V-ATPase and ClC-7 potently inhibited both acidification and resorption, as expected. We here show evidence that acidification of the resorption lacuna is mainly mediated by V-ATPase and ClC-7. Furthermore, a group of other ion transporters, including carbonic anhydrase II, the NHEs, and potassium-chloride cotransporters, are all involved in resorption but do not seem to directly be involved in acidification of the lysosomes.

Originalsprog Engelsk
Tidsskrift Calcified tissue international
Vol/bind 83
Tidsskriftsnummer 3
Sider (fra-til) 230-42
Antal sider 13
ISSN 0171-967X
DOI
Status Udgivet - sep. 2008

Muskelstyrken hos patienter med fibromyalgi. Et litteraturstudie

Dombernowsky, T., Dreyer, L., Bartels, E. M. & Danneskiold-Samsøe, B. 2008 I : Ugeskrift for Laeger. 170, 4, s. 217-24 8 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

Do patients with fibromyalgia (FM) have reduced muscular strength? We examined 22 articles and conclude from the results of these that FM patients have reduced muscular strength in their hands and quadriceps. The material also suggests generalised reduced muscular strength. However, the studies have several methodological shortcomings and future studies should be carefully designed with respect to patients as well as the control group and should be larger. To avoid CNS influence from e.g. fatigue and pain, muscular electro-stimulation may be used to ensure that the actual maximal muscular strength is also measured.
Originalsprog Dansk
Tidsskrift Ugeskrift for Laeger
Vol/bind 170
Tidsskriftsnummer 4
Sider (fra-til) 217-24
Antal sider 8
ISSN 0041-5782
Status Udgivet - 2008

Nordic Walking does not reduce the loading of the knee joint

Hansen, L., Henriksen, M., Larsen, P. & Alkjaer, T. aug. 2008 I : Scandinavian journal of medicine & science in sports. 18, 4, s. 436-41 6 s.

Publikation: Bidrag til tidsskriftTidsskriftartikelForskningpeer review

The use of Nordic Walking (NW) as a rehabilitation modality has increased considerably. NW (walking with poles) is advocated as a healthy physical activity that reduces the load on the knees. Few studies using the techniques of NW exist, and the findings are contradictory. The aim of this study was to investigate whether NW reduces the loadings upon the knee joint compared with walking without poles (NP). Seven experienced female NW instructors volunteered. Three-dimensional gait analyses were performed. Internal flexor and extensor joint moments were calculated using an inverse dynamics approach and the knee joint compressive forces were calculated. No differences in compression or shear forces between NW and NP were found. The peak knee flexion angles were larger during NW (-32.5+/-6.0 degrees) compared with NP (-28.2+/-4.2 degrees). The hip range of motion (ROM) was significantly increased during NW (64.4+/-10.2 degrees) compared with NP (57.8+/-9.7 degrees); no differences in the knee and ankle joint ROM were observed. The changes in the joint angles were not followed by changes in the joint dynamics. The present study does not support the statement that NW reduces the load on the knees.

Originalsprog Engelsk
Tidsskrift Scandinavian journal of medicine & science in sports
Vol/bind 18
Tidsskriftsnummer 4
Sider (fra-til) 436-41
Antal sider 6
ISSN 0905-7188
DOI
Status Udgivet - aug. 2008

PURPOSE: The amount of colour Doppler activity in the inflamed synovium is used to quantify inflammatory activity. The measurements may vary due to image selection, quantification method, and point in cardiac cycle. This study investigated the test-retest reliability of ultrasound colour Doppler measurements in the wrist of patients with rheumatoid arthritis (RA) using different selection and quantification methods.

MATERIALS AND METHODS: 14 patients with RA had their wrist scanned twice by the same investigator with an interval of 30 minutes. The images for analysis were selected either by the anatomical position only or by the anatomical position with maximum colour Doppler activity. Subsequently, the amount of colour Doppler was measured in an area defined by either the synovial tissue or by specific anatomical structures surrounding the synovial tissue.

RESULTS: The best test-retest reliability was obtained when the images were selected guided by colour Doppler and the subsequent quantification was done in an area defined by anatomical structures. With this method, the intra-class coefficient ICC (2.1) was 0.95 and the within-subject SD (SW) was 0.017, indicating good reliability. In contrast, poor test-retest reliability was found if the images were selected by anatomical position only and the quantification was done in an area defined by the synovial tissue (ICC [2.1] = 0.48 and SW = 0.049).

CONCLUSION: The study showed that colour Doppler measurements are reliable if the images for analysis are selected by anatomical position in combination with colour Doppler activity and the subsequent analysis is performed in an area defined by anatomical structures.

Originalsprog Engelsk
Tidsskrift Ultraschall in der Medizin (Stuttgart, Germany : 1980)
Vol/bind 29
Tidsskriftsnummer 4
Sider (fra-til) 393-8
Antal sider 6
ISSN 0172-4614
DOI
Status Udgivet - aug. 2008

Pages